FGSC #2205

Reporting Genes: pyr-1;uc-3

Species: crassa

Allele: H263;RW203

Depositor: LGW

Linkage Group: IVR;--

Mating Type: a

Species Number: 10

ref1: Williams and Mitchell 1969. J. Bact. 100:383-389, https://doi.org/10.1128/jb.100.1.383-389.1969

Genes

Locus	Cultural Requirements	Link Group	Type
pyr-1	IVR. Right of psi (4%) and T(ALS159) (808, PB). Left of pdx-1 (<1 to 10%) (692). (482) Requires uracil or other pyrimidine. Lacks dihydroorotate dehydrogenase activity (133, 134) (Fig. 20). All ascospores from pyr-1 x pyr-1crosses are white if cross is on medium containing 0.1 mg of uracil per ml; they are black if the cross is on medium containing 1.0 mg/ml (632).	IVR	B
uc-3	Unmapped. Thymidine salvage pathway defect (Fig. 23). Unable to use thymidine, thymine, or hydroxy-methylcytosine, but can use deoxyuridine, 5-formyluracil, uracil, or uridine to support growth of pyr-4 when uc-1 is also present. Excretes thymine into the medium when grown in the presence of thymidine (1141). Lacks thymine 7-hydroxylase, which catalyzes the reactions thymine -> 5-hydroxymethyluracil -> 5-formyluracil -> uracil-5-carboxylic acid; apparently, there is an alternate enzyme for 5-formyluracil -> uracil-5-carboxylic acid, which explains the growth on 5-formyluracil (616, 977).		B

Neurospora Crassa Wikipedia