FGSC #2203

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Reporting Genes: pyr-4 uc-1;uc-2

Species: crassa

Allele: 36601 RW57;RW135

Mutagen: UV

Depositor: LGW

Linkage Group: IIL II;I

Mating Type: A

Species Number: 10

Genetic Background: M

ref1: Williams and Mitchell 1969. J. Bact. 100:383-389, https://doi.org/10.1128/jb.100.1.383-389.1969

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
pyr-4	IIL. Right of het-c (1%), T(P2869), and cys-3 (18 to 21%). Left of ro-3 (1 to 2%) (721, 816, PB). (812). Requires uracil or other pyrimidine. Lacks orotidine 5'-monophosphate decarboxylase (133, 134, 841) (Fig. 20). Fertile crosses homozygous for pyr-4 can be made by using very high levels of uridine (15 to 20 mg/ml) (O.M. Mylyk, personal communication).	IIL	B
uc-1	II. Linked to pyr-4 (31%) (1139, 1141). Altered thymidine salvage pathway (Fig. 23). Able to use thymidine, thymine, 5-hydroxy-methyluracil, or 5-formyluracil as the sole pyrimidine source in germinating conidia of pyr-4 mutants, which are blocked in pyrimidine biosynthesis. (In uc-1+ background, pyr-4 can use these compounds only if a primer of uridine or cytidine is supplied [1141]). Causes elevated activities of enzymes which oxidatively demethylate thymine (420).	II	B
uc-2	I. Linked to mt (0/12 asci) (1139, 1141).Thymidine salvage pathway defect (Fig. 23). Unable to use thymidine or deoxyuridine, but can use thymine, 5-hydroxymethyluracil, 5-formyluracil, uracil, or uridine as the sole pyrimidine source for pyr-4when uc-1is also present (1141). Reduced activity of the 2'-hydroxylase reactions thymidine -> thymine ribonucleoside and deoxyuridine -> uridine (977).	I	B