FGSC #2202

Reporting Genes: ud-1;pyr-1

Species: crassa

Allele: RW433;H263

Mutagen: UV

Depositor: LGW

Linkage Group: VIIR;IVR

Mating Type: a

Species Number: 10

Genetic Background: SL

ref1: Williams and Mitchell 1969. J. Bact. 100:383-389, https://doi.org/10.1128/jb.100.1.383-389.1969

Genes

Locus	Cultural Requirements	Link Group	Type
pyr-1	IVR. Right of psi (4%) and T(ALS159) (808, PB). Left of pdx-1 (<1 to 10%) (692). (482) Requires uracil or other pyrimidine. Lacks dihydroorotate dehydrogenase activity (133, 134) (Fig. 20). All ascospores from pyr-1 x pyr-1crosses are white if cross is on medium containing 0.1 mg of uracil per ml; they are black if the cross is on medium containing 1.0 mg/ml (632).	IVR	B
ud-1	VIIR. Between met-7 (27%) and arg-10 (10%) (126). (463). Unable to use pyrimidine nucleosides to support the growth of a pyrimidine auxotroph (pyr-1) although any free pyrimidine base can be used. Probably defective in pyrimidine nucleoside transport (1141) (Fig. 23). Apparently also defective in purine nucleoside transport (224). Resistant to 5-fluorodeoxyuridine and 5-fluorouridine (126). Resistance is recessive in heterokaryons. Shows interallelic complementation (127). Scored by spotting conidial suspension on medium containing 4 x 10(-5)M 5-fluorodeoxyuridine, filter sterilized (463). Mutant gene CIF-dUrd 7, selected by resistance and called fdu-1 (463), is allelic (126). Uridine uptake is decreased by good nitrogen sources and by nit-2 (128).	VIIR	B

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