FGSC #2201

Reporting Genes: uc-5 pyr-1

Species: crassa

Allele: RW399;H263

Mutagen: UV

Depositor: LGW

Linkage Group: IVR IVR

Mating Type: a

Species Number: 10

Genetic Background: SL

ref1: Williams and Mitchell 1969. J. Bact. 100:383-389, https://doi.org/10.1128/jb.100.1.383-389.1969

Genes

Locus	Cultural Requirements	Link Group	Type
pyr-1	IVR. Right of psi (4%) and T(ALS159) (808, PB). Left of pdx-1 (<1 to 10%) (692). (482) Requires uracil or other pyrimidine. Lacks dihydroorotate dehydrogenase activity (133, 134) (Fig. 20). All ascospores from pyr-1 x pyr-1crosses are white if cross is on medium containing 0.1 mg of uracil per ml; they are black if the cross is on medium containing 1.0 mg/ml (632).	IVR	B
uc-5	IV. Right of cot-1 (32%) (126). (1139, 1141). Apparently defective in transport of pyrimidine bases (Fig. 23). Unable to use any free pyrimidine base to support the growth of the pynmidine auxotroph pyr-1, although it can use both ribose- and deoxyribose-nucleosides (1141). Resistant to 5-fluorouracil (126). The pyr-1 uc-5 double mutant has been used to study uptake inhibition of structural analogs (225). Selected by inability to use uracil to supplement pyr auxotrophs, or as 5-fluorouracil resistant on ammonia-free minimal medium (126). Uracil uptake is decreased by NH3 (629) or by other good nitrogen sources, and by a nit-2 mutation (128). Called tru.	IV	B

Neurospora Crassa Wikipedia