FGSC #1998

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Reporting Genes: nuc-1;nuc-2

Species: crassa

Allele: T28-M1;T28-M2

Depositor: TI

Linkage Group: IR;IIR

Mating Type: A

Species Number: 10

ref1: Ishikawa et al. 1969. Genetics 63:75-92, https://www.genetics.org/content/genetics/63/1/75.full.pdf

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
nuc-1	IR. Right of T(AR173) and his-2 (<1%). Left of lys-4(1%). (514) nuc-1mutants (other than nuc-1c) are unable to use RNA or DNA as a phosphorus source (450, 514). Defective in production of repressible alkaline and acid phosphatases (671, 1077). Several nucleases are absent or reduced (449). nuc-1 is epistatic to both pconc and pregc (671) and to pgovc (665). Scored on low-phosphate medium by a staining reaction with alpha-naphthyl phosphate plus diazo blue B (397, 1077), by failure to grow on minimal medium altered so that 0.1 g of RNA or DNA per liter is substituted for the inorganic phosphate source (514, 538), or by failure to grow on low-phosphate medium at a pH above 7 (R.L. Metzenberg, personal communication). nuc-1c is constitutive for alkaline phosphatase synthesis and maps very close to nuc-1. nuc-1c acts only if it is cis to normal nuc-1. In duplications, nuc-1c is dominant to nuc-1+, which is dominant to nuc-1. nuc-1c is epistatic to nuc-2(670). nuc-1c is scored on high-phosphate medium by a staining reaction with alpha-naphthyl phosphate plus diazo blue B (397, 1077) or by suppression of the nuc-2 phenotype on low-phosphate medium at high pH (670). Used to study phosphate transport (624). For regulation model see references 665 and 670.	IR	B
nuc-2	IIR. Between the T(NM177) breakpoints; hence, right of aro-3. Left of preg(1 to 2%) and pe (4%). Probably allelic with pcon (0/854) (593, 671). (514) Unable to use RNA or DNA as a phosphorus source (514). Defective in production of repressible alkaline and acid phosphatases (671, 1077). Several nucleases absent or reduced (449). Interaction with other phosphate regulatory genes (665). Recessive to nuc+ in partial diploids and heterokaryons (671). Not defective in nuh function (538). Scored on low-phosphate medium by a staining reaction with alpha-naphthyl phosphate plus diazo blue B (397, 1077), by failure to grow on minimal medium altered so that 0.1 g of RNA or DNA per liter is substituted for the inorganic phosphate source (514, 538), or by failure to grow on low-phosphate medium at a pH above 7 (R.L. Metzenberg, personal communication). Used to study phosphate transport (624). For a regulation model, see references 665 and 670. See pcon.	IIR	B