FGSC #10457

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Reporting Genes: csp-1 his-3+::frq^P-luc; chol-1 ras-1^bd frq7

Species: crassa

Alternate Strain Number: JCD 533-29

Depositor: JCD

Mating Type: A

Species Number: 10

ref1: Shi et al PNAS 104:20102-20107, https://doi.org/10.1073/pnas.0706631104

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
chol-1	IVR. Linked to ad-6 (1%). probably to the right (633). (482) Requires choline (470). Also uses mono- or dimethylaminoethanol (468) (Fig. 12). Deficient in S-adenosylmethionine:phosphatidylethanolamine-N-methyl transferase (222, 924). Abnormal colonial morphology on limiting choline (222). Colonies from single conidia on minimal agar medium resemble inhibited A/a duplications, with swollen hyphae and darkening in the presence of phenylalanine plus tyrosine (D.D. Perkins, unpublished data). Abnormal phospholipid composition on limited concentrations of supplement (501). Best scored late on minimal medium. Grows slightly and then stops (D.D. Perkins, unpublished data). Used to study inhibition of cytochrome-mediated respiration and of conidiation when lecithin is depleted by choline starvation (534, 535). Initial allele called 34486.	IVR	B
csp-1	IL. Between arg-3 (1%) and the T(39311) right breakpoint (972, PB). Conidia fail to separate and become airborne. Photograph (972). Recessive. Cultures on agar readily scored by the "tap test." In water, conidia are freed at 1/10 the wild-type concentration (972). Used in connection with bd for study of circadian rhythms (e.g., reference 114). Useful in student laboratories to avoid contamination (966). Carotenoids tend to be yellowish in young cultures (PB).	IL	B