FGSC #1513

Reporting Genes: cot-2

Species: crassa

Allele: R1006(t)

Alternate Strain Number: 5168

Mutagen: UV

Depositor: DDP

Linkage Group: VR

Mating Type: A

Species Number: 10

Genetic Background: SL2

Opposite Mating Type: 2263

ref1: Garnjobst and Tatum 1967. Genetics 57:579-604, https://doi.org/10.1093/genetics/57.3.579

ref2: Morgan et al 1967 Genetics 57:605-612 XM86, PMID: 4868675, https://www.genetics.org/content/genetics/57/3/605.full.pdf

Genes

Locus	Cultural Requirements	Link Group	Type
cot-2	VR. Right of pk (8%) and ser-2 (5%). Left of ad-7(4%) (156, 818). (698) Recombines with inv (5%) (315).Small colonies at 34°C but fully viable. Growth and morphology nearly normal at 25°C but not completely so (382). Makes altered invertase; it is not clear whether cot-2 is the structural gene for a second subunit or whether cot-2affects structure indirectly, e.g., by altering the carbohydrate moiety (315). See inv. Reduced amount of cell wall peptides (1165). Ascospores are normal in heterozygous crosses, but are round in homozygous cot-2 x cot-2crosses (59). Some cot-2 strains carry mei-3, which was found in the original cot-2 strain (757); however, most strains used by Eggerding et al. (315) are free of it, and mei-3 cannot be responsible for the effects on invertase (D. Newmeyer, unpublished data).	VR	B

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