FGSC #1501

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Reporting Genes: leu-4;leu-1

Species: crassa

Allele: FLR92;D221

Alternate Strain Number: FLR92-1-216A

Mutagen: UV

Depositor: SRG

Linkage Group: IL;IIIR

Mating Type: A

Species Number: 10

Genetic Background: M

ref1: Gross, S.R. PNAS 54:1538 1965 XG62, https://doi.org/10.1073/pnas.54.6.1538

ref2: Calvo & Gross 1970 Meth Enzymology 17:791

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
leu-1	IIIR. Between ad-4 (1 to 5%) and his-7 (8%) (219, 578, 815, 1052). (868)Requires leucine (867, 868). Lacks beta-isopropylmalate dehydrogenase (426) (Fig. 15). Accumulates alpha-isopropylmalate and beta-isopropylmalate (427). Synthesis of the enzyme also requires the function of regulatory gene leu-3+ and the presence of alpha-isopropylmalate, which acts as inducer (427). Resistant to aminotriazole (D. D. Perkins, unpublished data). Female sterile (O. M. Mylyk, personal communication). Used to study reversion and competition in heterokaryons (901).	IIIR	B
leu-4	IL. Right of T(OY321); hence, of cyt-1. Left of cys-5(<<1%) (1125; D.D. Perkins, N.B. Raju, and E.G. Barry, in preparation). (429)Requires leucine, Structural gene for alpha-isopropylmalate synthetase (426, 427, 432) (Fig. 15). Feedback-negative mutants (426, 427). Hybrid synthetases with altered properties (432). Complementation between alleles (432, 1125).	IL	B