FGSC #1402

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Reporting Genes: le-1;pe fl

Species: crassa

Allele: S4355;Y8743m L

Alternate Strain Number: S4355le a

Mutagen: UV

Depositor: ELT

Linkage Group: IVR;IIR IIR

Mating Type: a

Species Number: 10

Genetic Background: L

ref1: Garnjobst and Tatum 1967. Genetics 57:579-604, https://www.genetics.org/content/genetics/57/3/579.full.pdf

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
fl	IIR. Between ace-1 (5 to 11%) and trp-3 (3%) (816, PB). (613)No macroconidia (609). Highly fertile (612). Used routinely as the female parent in tests for chromosome rearrangements and for mating type (e.g., reference 801). The flsingle mutant produces few microconidia when dry; when wetted, sufficient microconidia are produced to have been used in early irradiation and mutation studies (614, 915); large numbers can be obtained under certain conditions; see reference 893. pe fl (46, 700) and fl;dn (806) double mutants produce abundant microconidia; the latter combination is highly fertile when homozygous. Photograph of microconidial formation (774); see also reference 893. Nuclear numbers in microconidia (46, 64, 478). Wall analysis (207). Immunoelectrophoretic pattern (784). Paradoxical high alcoholic glycolysis on nitrate medium (80). Deficiency of isocitrate lyase on acetate medium; see citations in reference 1088. When fl A and fl a strains are inoculated separately on crossing medium in plates, a double line of perithecia forms where they meet, similar to that accompanying barrage in Podospora (410, 414). fl ascospores from certain fl x fl+ crosses often germinate spontaneously (1127; N. B. Raju, personal communication). Allele C-1835 was called acon (717, 812).	IIR	B
le-1	IVR. Linked to pan-1 (1 to 2%) (382). Right of cot-1 (14%) (713, 716).Autonomous ascospore lethal with colonial growth. Ascospores are black but mostly fail to germinate. A few do so after special treatment (382). Aconidial colonial growth with dense granular aerial mycelium, turning brown with age (713, 716). Photographs (382, 716). Reduced amount of cell wall peptides (1165). Alleles B55 and S4355 of reference 382 were presumed to be allelic with similar mutations called col-le-1(CM3) and (col-le-2) by (713, 716), but direct tests were not made.	IVR	B
pe	IIR. Between nuc-2 (4%) and arg-12 (1 to 5%) (593, 816). (613) Peach-colored conidia and short hyphae formed, more uniformly than by the wild type, as a lawn close to surface of agar. Distinctive morphology (46, 613). Added arginine increases macroconidiation and tends to obscure scoring of peat 25 C, but not at 39°C. pe single mutants produce both macro- and microconidia. pe fl double mutants produce abundant grey microconidia and no macroconidia (46, 700) (see fl). See col-1, col-4, and references 415 and 416 for interactions with other genes. Called m (microconidial) or pem in some contexts.	IIR	B