FGSC #1357

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Reporting Genes: cot-4 inl

Species: crassa

Allele: R2101(t) 89601

Alternate Strain Number: R2101-2(2-1)

Mutagen: UV

Depositor: ELT

Linkage Group: VR VR

Mating Type: A

Species Number: 10

Genetic Background: M

ref1: Garnjobst and Tatum 1967. Genetics 57:579-604

ref2: Morgan et al Genetics 57:605-612 1967 XM86

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Genes

Locus	Cultural Requirements	Link Group	Type
inl	VR. Between pho-3 (3 to 4%) and pab-1 (1 to 10%). Right of al-3 (362, 397, 1036). (482)Requires inositol (65). Lacks D-myoinositol-1-phosphatase (1142). Lack of glucocycloaldolase found by Pina and Tatum (826) is attributed by Williams (1142) to drastic repression of glucocycloaldolase by the concentration of inositol used for growth. Growth is colonial on low levels of inositol (367). Tends to extrude dark pigment into the medium when grown on suboptimal inositol. Composition of phospholipids and cell walls is abnormal on limiting inositol (367, 439, 440, 501). Inhibited by hexachlorocyclohexane (366, 457, 931). Conidia are subject to death by unbalanced growth on minimal medium (1028, 1033), a property exploited for mutant enrichment ("inositol-less death") (606, 647) because double mutants are at a selective advantage. Heat-sensitive allele 83201 is especially useful for mutant enrichment (832, 1043). Used in the first experiments reporting transformation of Neurospora by N. crassaDNA (677, 679) and reported to be efficient as a recipient in absence of inositol (1162). Used to study glucose (917) and sulfate (641) transport systems. Used extensively for studying induced reversion (392). Used for studying the mechanism of inositol-less death (647, 702), mutagenicity of ferrous ions, and regulation of mitochondrial membrane fluidity; for a review, see reference 702. Spontaneous reversion rates (386). Allele-specific partial suppressor (390). Allele 46802 is nonrevertable and inseparable from translocation 46802 (386, 808). Strains carrying heat-sensitive allele 83201 show slow semicolonial growth in liquid minimal medium at 25°C (641), but look normal on slants (D.D. Perkins, unpublished data). Strains carrying allele 89601 contain cross-reacting material (1183). Mutant gene exo-1 is present in the inl(89601) a stock FGSC 498 and may, therefore, be present in stocks of mutants derived by inositol-less death. (See references 194, 325, and 1027). Called inos.	VR	B
cot-4	VR. Right of ilv-1 (8%) and rol-3 (5%). Left of inl(10%) (698). Not allelic with sp (11%) (PB).Small colonies at 34°C, spreading at 25°C (382). Morphology at 25°C resembles that of the mutant sp, with late-forming blooms of conidia on aerial hyphae, but sp is not heat sensitive. Good female fertility, but no perithecia in homozygous cot-4 crosses (PB).	VR	B