FGSC #1294

Reporting Genes: phe-1

Species: crassa

Allele: NM160

Alternate Strain Number: 2551-6

Mutagen: UV

Depositor: DDP

Linkage Group: IL

Mating Type: A

Species Number: 10

Genetic Background: SL2

Opposite Mating Type: 1295

ref1: Perkins et al Genetica 1969 40:247-278, https://doi.org/10.1007/bf01787357

Genes

Locus	Cultural Requirements	Link Group	Type
phe-1	IL. Right of In(H4250) and suc (<1%). Left of ad-5(816; H.B. Howe, Jr., personal communication). (48) [Duplications from In(H4250) x phe-1 crosses are phe-, unlike duplications from T(39311). The contrary statement on p. 268 of reference 816 is a misprint.] Originally reported to grow on phenylalanine, other aromatic amino acids, leucine, or ethyl acetoacetate, with phenylalanine being most effective; several other acids gave smaller responses (48). Utilization of phenylalanine and other compounds varies for different isolates and on different carbon sources; glycerol or ribose is preferable to sucrose (521-523, 753). Strains carrying allele NM160 do not use phenylalanine but grow well on tyrosine or leucine (816; A.G. De Busk, personal communication), at least with the strains and carbon source used. The mutant phe-1 is inhibited by basic amino acids on low-phenylalanine or leucine medium (48, 521). Growth on beta-labeled leucine or beta-labeled phenylalanine showed that neither compound is converted to the other (45). Called phen-1. Allele NM160 originally called tyr(NM160) (316).	IL	B

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