FGSC #1289

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Reporting Genes: Not available; cys-3 fl

Species: crassa

Allele: P22 P

Alternate Strain Number: 2094

Depositor: DDP

Linkage Group: IIL IIR

Mating Type: a

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
cys-3	IIL. Right of pi (4%). Left of T(AR18) and pyr-4 (18 to 21%) (721, 808, 816).Uses sulfite, cysteine, or methionine; little or no response to thiosulfate (640, 721). Regulator of genes of sulfur uptake and metabolism (e.g., sulfate permease, aryl sulfatase, choline sulfatase) (284, 640, 667). Grows well on methionine. Resistant to chromate (640). Used extensively for studying regulation; for a review, see reference 642. cys-3 ascospores darken slowly or not at all, even when a cys-3 strain is the fertilizing parent and when a strain carrying heat-sensitive allele NM27t is crossed at 25°C, the permissive temperature for growth. Adding methionine to crossing medium promotes darkening but fails to give good recovery of cys-3 progeny. Recovery of a few percent cys-3 progeny is possible in well-aged crosses. (721, PB). cys-3 can be used effectively as an autonomous ascospore color mutant for demonstrating segregation patterns in asci (811; see reference 858 for photograph; see Ascospore color mutants).	IIL	B
fl	IIR. Between ace-1 (5 to 11%) and trp-3 (3%) (816, PB). (613)No macroconidia (609). Highly fertile (612). Used routinely as the female parent in tests for chromosome rearrangements and for mating type (e.g., reference 801). The flsingle mutant produces few microconidia when dry; when wetted, sufficient microconidia are produced to have been used in early irradiation and mutation studies (614, 915); large numbers can be obtained under certain conditions; see reference 893. pe fl (46, 700) and fl;dn (806) double mutants produce abundant microconidia; the latter combination is highly fertile when homozygous. Photograph of microconidial formation (774); see also reference 893. Nuclear numbers in microconidia (46, 64, 478). Wall analysis (207). Immunoelectrophoretic pattern (784). Paradoxical high alcoholic glycolysis on nitrate medium (80). Deficiency of isocitrate lyase on acetate medium; see citations in reference 1088. When fl A and fl a strains are inoculated separately on crossing medium in plates, a double line of perithecia forms where they meet, similar to that accompanying barrage in Podospora (410, 414). fl ascospores from certain fl x fl+ crosses often germinate spontaneously (1127; N. B. Raju, personal communication). Allele C-1835 was called acon (717, 812).	IIR	B