FGSC #1274

Reporting Genes: cys-5

Species: crassa

Allele: NM44

Alternate Strain Number: 1073

Mutagen: UV

Depositor: NEM

Linkage Group: IL

Mating Type: a

Species Number: 10

Genetic Background: E

ref1: Murray, N.E. 1965 Genetics 52:801 XM109, https://www.genetics.org/content/genetics/52/4/801.full.pdf

Genes

Locus	Cultural Requirements	Link Group	Type
cys-5	IL. Between leu-4 and ser-3 (0.1%) (816, 1125). (N.H. Horowitz, cited in references 721 and 815) Probably a locus distinct from cys-11, with the order leu-3 cys-5 (<1%) cys-11 mt in a cross showing no negative interference (723).Uses sulfite, thiosulfate, cysteine, or methionine (721). Lacks 3'-phosphoadenosine-5'-phosphosulfate reductase (F.-J. Leinweber, cited in references 721 and 723). Enzymatically distinct from cys-11 (adenosine 5'-triphosphate sulfurylase), which it complements in heterokaryons (721, 723; F.-J. Leinweber, cited in references 721 and 723). Leaky, but not so as to interfere with scoring. Ascospores may be oozed from perithecial beaks rather than shot. For good recovery of cys-5 progeny, crossing media should be supplemented even when the protoperithecial parent is cys+; otherwise cys-5ascospores may fail to blacken. cys(NM86) and cys(85518), initially listed as cys-5 alleles, are now designated cys-11.	IL	B

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