FGSC #1224

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Reporting Genes: gluc-1;cot-1

Species: crassa

Allele: CM26(3-8);C102(t)

Alternate Strain Number: CM26(3-8)a

Mutagen: UV

Depositor: BME

Linkage Group: --;IVR

Mating Type: a

Species Number: 10

Genetic Background: M

ref1: Eberhart et al J. Bact. 87:761-70 1964 XE4, https://doi.org/10.1128/jb.87.4.761-770.1964

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
gluc-1	IIIR. Linked to dow (10%) (B.M. Eberhart, personal communication).Activity of the thermostable aryl-beta-glucosidase reduced to 10% of the wild type (300) by one allele, and to <1% in a second-step mutant then called gluc-2, which showed 0/200 recombination with the original mutation and is probably allelic (299). Low activity is dominant in heterokaryons (630). Selected by the p-nitrophenyl glucoside staining reaction (297). Scored by breakdown of the beta-glucoside esculin (0.01%) as measured by fluorescence at pH 5.5 (300), or by precipitation of ferric ammonium citrate (0.1%) by esculetin (2 days, 25 C) (B.M. Eberhart, personal communication).	IIIR	B
cot-1	IVR. Between pan-1 (2%) and his-4 (1 to 6%) (692, 812, 816). Extremely colonial at 34°C, but completely normal growth, morphology, and fertility at 25°C and below. Linear growth is maximum at 24°C (374). Becomes colonial at 32°C; colonies from ascospores or conidia are viable and continue to grow slowly with dense branching, but do not conidiate. They quickly resume normal growth when shifted to a permissive temperature (692, 1068). Recessive in duplications (808); apparent dominance in heterokaryons (374) may have resulted from a shift in nuclear ratios. Used in studies of septation and branching (202), growth-inhibiting mucopolysaccharide (878, 879), and sulfate transport (641). Cell wall analysis (374). Growth is stimulated by lysine or arginine (0.1 mM) on glucose media at high temperatures (615). Because of high viability and tightly restricted growth at restrictive temperatures and normality at 25°C, cot-1 mutants have valuable technical applications. For example, crosses homozygous for cot-1 have been used in combination with sorbose for experiments with rec genes, where high-density ascospore platings are required for precise quantitative analysis of intralocus recombination (e.g., references 165, 997, and 1070). In another application, when shifted up after initial growth at the permissive low temperature, cot-1hyphae assume a "bottle brush" appearance with small side branches (692). This has been used to select uvs mutants by subsurface survival on UV-irradiated plates containing p-aminobenzoic acid (938; D.E.A. Catcheside, personal communication). cot-1 conidia or ascospores from cot-1 x cot-1crosses are used for replication in a protocol involving transfer by filter paper (615). For suppressors of cot-1, see gul.	IVR	B