FGSC #1114

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Reporting Genes: suc;ma-1;ma-2

Species: crassa

Allele: 46005;M20;M24

Alternate Strain Number: (M20 x 24-1-

Depositor: KDM

Linkage Group: IR;--;--

Mating Type: a

Species Number: 10

ref1: Munkres et al. 1965 Arch Biochem Biophys 109:397, https://doi.org/10.1016/0003-9861(65)90379-6

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ma-1	Unmapped. Probably in a left arm, any of III to VII.Unable to use malate as a carbon source when the tricarboxylic acid cycle is blocked by a suc (pyruvate carboxylase) mutation. Altered mitochondrial malate dehydrogenase. Scorable only in sucmutants (703, 706, 709).		B
ma-2	IIR. Between un-20 an ace-1. Unlinked to ma-1. Unable to use malate as a carbon source when the tricarboxylic acid cycle is blocked by a suc (pyruvate carboxylase) mutation. Altered mitochondrial malate dehydrogenase. Scorable only in suc mutants (703, 706, 709).	IIR	B
suc	IL. Right of acr-3 (2%). Left of In(H4250) and phe-1(1%) (578, 816). (482). Uses acetate, succinate, or any of numerous related compounds (608). Most but not all strains grow better on acetate than on succinate (576). Lacks pyruvate carboxylase activity (72, 578, 1032). Numerous alleles are CO2 remediable (108). Leaky, but less so on higher ammonium concentrations (1031). A special medium has been devised for selective plating (K.D. Munkres, cited in reference 816, p. 248). Most ascospores are poorly pigmented in homozygous suc x succrosses. Allele KG163 shows greatly reduced recombination in the region between leu-4 and suc, suggesting inversion heterozygosity (576). suc would be named ace-6 except for priority of nomenclature (578).	IL	B