FGSC #1080

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Mutant Type

Genus: N

reporting_genes: Not available; T(I;VII)17084 thi-1 T(IV;VI)45502 pyr-3

species: Neurospora crassa

allele: 45502

stock: 1746-159

glasgow:

mutagen:

Depositor: DDP

Link Group: IR;VIIL + IVR;VIR

MT: a

Species No: 10

gene_back:

oppmt: 1079

trans:

ref1:

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-1080

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-1080 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
T(I;VII)17084	Translocation		B
thi-1	IR. Right of the T(4540) right breakpoint and cys-9 (13%). Left of T(NM103), T(ALS182), and met-6 (7 to 14%) (721, 808, 816, 1091). (482). Uses thiamine or precursors pyrimidine plus thiazole (1059). Adaptation to growth on minimal medium occurs after a lag; growth tests should, therefore, be scored early. Adaptation is not carried over via ascospores, conidia, or small mycelial fragments. Adaptive growth is paralleled by attainment of wild-type thiamine pyrophosphate and carboxylase levels. Apparently concerns utilization of intact thiamine rather than its biosynthesis. (302, 303). Allele 17084 is inseparable from translocation T(IR;VII)17084 (808).	IR	B
pyr-3	IVR. Right of the T(NM152) left breakpoint and of T(S1229); hence, right of arg-14. Left of his-5 (1%) (238, 808). (482) Requires uracil or other pyrimidine (683). Growth inhibited by purine nucleosides and nucleotides (825). Structural gene for pyrimidine-specific carbamyl phosphate synthase (CPS) and aspartate carbamyl transferase (ACT; also abbreviated ATC) (456, 850) (Fig. 20). Mutants may lack either or both activities, e.g., those carrying alleles KS43 (CPS+ ACT-), KS20 (CPS- ACT+), and KS11 (CPS- ACT-) (1140). Unlike Saccharomyces, no feedback-insensitive CPS+ ACT+ mutants of Neurosporahave been discovered (A. Radford, unpublished data). Some mutants have kinetically altered aspartate carbamyl transferase (456, 880). Used extensively for studies of channeling and relation of gene structure to the two enzyme activities (236). Normally, carbamyl phosphate produced by pyr-3+ is used solely for pyrimidine synthesis, and carbamyl phosphate produced by arg-2+ and arg-3+ is used for arginine synthesis, the enzymes being in different organelles; however, a deficiency of the next enzyme in either pathway permits overflow of carbamyl phosphate into the other pathway (reviewed in reference 236). Hence, CPS- ACT+ alleles are suppressed by arg-12s (246), and CPS+' ACT- alleles can be selected as suppressors of arg-2and arg-3 (658, 887, and references therein). Some of the CPS+ ACT- mutations, called pyr-su-arg, suppress the arginine requirement but retain enough aspartate carbamyl transferase activity that they have no detectable pyrimidine requirement (877, 881). arg-13, arg-4, arg-5, arg-6, and am partly suppress CPS- ATC+ alleles (see reference 660). Fine-structure map (851, 1050). Fertility of interallelic crosses is variable and often very poor (658). Complementation between CPS- ACT+ and CPS+ ACT- mutants (246) and between some pairs of CPS+ ACT- mutants is good; otherwise, complementation is poor (849, 1159). Complementation maps (658, 849, 877, 1159). Mutational analysis (852). Direction of translation, based on enzyme types of polar mutants, is from CPS to ACT (850). Allele 37815(t) is heat sensitive (34°C versus 25 C) (68). Allele 1298 is C02 remediable (191, 192). Strain KS12, a pyr-1 pyr-3 double mutant, was originally called pyr-5 (see reference 346). The different classes of pyr-3 alleles have been called M (CPS-P-less), N (ACT-less), and MN (lacks both activities).	IVR	B
T(IV;VI)45502	Translocation		B