FGSC #994

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Reporting Genes: caf-1

Species: crassa

Allele: KH101(r)

Alternate Strain Number: 507

Mutagen: S

Depositor: KSH

Linkage Group: VL

Mating Type: a

Species Number: 10

Genetic Background: SL

Opposite Mating Type: 993

ref1: Hsu 1962. Genetics 47:961 (Abstr.), https://www.genetics.org/content/genetics/47/8/937.full.pdf

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
caf-1	VL. Right of T(OY321) (11%), T(AR30) (19%), and. hence, of NO. Left of T(AR33)(5 to 12%) and lys-1 (4 to 14%). (817, PB; K.S. Hsu, unpublished data) Resistant to caffeine (494). Resistance is dominant in duplications from T(AR33) (817). Scoring is clear at 25°C and poor at 34°C on slants with 2 or 2.5 mg of caffeine per ml of minimal medium without sorbose (R.L. Metzenberg, personal communication). Also readily scorable by using conidial suspensions spotted on plates containing 2 mg of caffeine per ml of minimal sorbose medium (PB).	VL	B