FGSC #780

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Reporting Genes: his-1 met-3

Species: crassa

Allele: K744 36104

Alternate Strain Number: 2864, ATCC 14768

Depositor: DGC

Linkage Group: VR VR

Mating Type: a

Species Number: 10

ref1: Catcheside 1960 Proc Roy Soc London 153:179-194, https://doi.org/10.1098/rspb.1960.0095

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
his-1	VR. Right of ure-1 (1%). Left of pho-2 (3%), al-3, and inl (1 to 10%) (397, 570, 578, 1036). (434)Requires histidine (434). Accumulates imidazole glycerol phosphate. Lacks imidazole glycerol phosphate dehydrase (24, 25) (Fig. 14). Intralocus complementation (162). Recombination between his-1 alleles is controlled by rec-1(172, 520, 1070). Initial his-1 allele called C84.	VR	B
met-3	VR. Right of trp-5 (4%) and pab-1 (1%). Left of pk(1%) (6, 296, 362, 1036). (125) Uses methionine, homocysteine, or cystathionine (469). Lacks cystathionine-gamma-synthase (547) (Fig. 17). This enzyme is also lacking in the mutant met-7(547). The enzyme is activated by methyl tetrahydrofolate and feedback inhibited by S-adenosylmethionine (547, 965).	VR	B