FGSC #636

Reporting Genes: un-3

Species: crassa

Allele: 55701(t)

Alternate Strain Number: N104-16A

Mutagen: X

Depositor: DNP

Linkage Group: IL

Mating Type: A

Species Number: 10

Genetic Background: M

Opposite Mating Type: 4529

ref1: Howe 1961. Nature 190:1036, https://doi.org/10.1038/1901036a0

Genes

Locus	Cultural Requirements	Link Group	Type
un-3	IL. Right of In(NM176); hence, right of ser-3 (1%) (1093). Left of mt(0.04 to 0.1%) (488, 758). Closest bracketing marker left of mt (482). Unknown function. Heat sensitive (484). Growth drops off sharply between 28.5°C and 30°C (R.L. Metzenberg, personal communication). Multiply transport deficient at permissive temperatures with increased fragility of protoplasts (543); reduced rate of uptake of citrulline (1075) and aspartate (543, 1149). Resistant to ethionine and to p-fluorophenylalanine at 25°C (542, 543). Used to tag mating type (487) and as a flanker in an attempt to resolve the mating type region by recombination (758). Strains with probable un-3alleles are selected as citrulline-resistant mutants of pyr-3 arg-12s; most mutants selected in this way show complementation between alleles (1075). A possible functional relation to mating type is discussed in reference 543, Growth at 25°C aided by 0.3 mg of sodium acetate per ml. May be scored by slow growth at 25°C if acetate is not added to minimal medium (487). Formerly called un(55701).	IL	B

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