FGSC #576

Mutant Type

Genus: N

reporting_genes: sfo nt

species: Neurospora crassa

allele: E18829(d) 65001

stock: E-18558-9(22-5)

glasgow:

mutagen:

Depositor: ELT

Link Group: VIIC VIIR

MT: A

Species No: 10

gene_back:

oppmt: 506

trans:

ref1:

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-576

Locus	Cultural Requirements	Link Group	Type
sfo	Linked to the centromere (<1%) (318), between thi-3 (6%) (874) and hlp-1(1 to 9%) (458). Requires sulfonamide at 35°C and is stimulated by sulfonamide at lower temperatures (319). Overproduces p-aminobenzoic acid; hence, growth is inhibited by exogenous p-aminobenzoic acid (1179). For sfo;pab-1 double mutant on minimal medium plus sulfonamide, p-amino-benzoic acid is stimulatory at very low concentrations but inhibitory at higher (319). For sfo;met-1 double mutant on minimal medium plus sulfonamide, methionine is stimulatory at very low concentrations but inhibitory at higher (1180). Best tested on solid minimal medium growth tubes at 35°C. Suppressor mutations occur frequently (318).	VII	B
nt	VIIR. Between arg-10 (2 to 12%) and sk (7 to 18%) (789). (874) Uses nicotinic acid. May respond also to tryptophan, phenylalanine, tyrosine, quinic acid, and precursors of nicotinic acid or tryptophan, or both, depending on genetic background (448, 760). Best supplemented with nicotinamide and scored as a nic mutant. Probably deficient in tryptophan pyrrolase (tryptophan 2,3-dioxygenase) (Fig. 18), but direct evidence is lacking because tryptophan oxygenase cannot be assayed in Neurospora (368). Kynurenine formamidase levels are normal (368). Pyridine nucleotide levels (111).	VIIR	B

Neurospora Crassa Wikipedia