FGSC #536

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Reporting Genes: al-2;pe;col-1

Species: crassa

Allele: 15300;Y8743m Y8743c

Alternate Strain Number: Y-8743-2-6A

Depositor: ELT

Linkage Group: IR;IIR;IVR

Mating Type: A

Species Number: 10

ref1: Barratt et al 1954 Adv Genet 6:1-93, https://doi.org/10.1016/s0065-2660(08)60127-3

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B
col-1	IVR. Linked to pan-1 (0/47 asci) and cot-1 (3%) (46, 374). Colonial morphology, with no macroconidiation. Growth cyclic at moderate temperatures; steady at 38°C. Double mutant col-1 cot-1 grows better than col-1 at 24°C and better than cot-1 at 38°C (374). The double mutant pe;col-1forms microconidia (46) and was used in early mutation studies, but col-4;pe fl was found to be better (386). It was found (416) that pe;col-1 strains form microconidia at 25°C but form macroconidia at 35°C. Cell wall analysis; photograph (278). col-5 and col-8 not tested for allelism.	IVR	B
pe	IIR. Between nuc-2 (4%) and arg-12 (1 to 5%) (593, 816). (613) Peach-colored conidia and short hyphae formed, more uniformly than by the wild type, as a lawn close to surface of agar. Distinctive morphology (46, 613). Added arginine increases macroconidiation and tends to obscure scoring of peat 25 C, but not at 39°C. pe single mutants produce both macro- and microconidia. pe fl double mutants produce abundant grey microconidia and no macroconidia (46, 700) (see fl). See col-1, col-4, and references 415 and 416 for interactions with other genes. Called m (microconidial) or pem in some contexts.	IIR	B