FGSC #485

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Reporting Genes: chol-1

Species: crassa

Allele: 34486

Alternate Strain Number: 34486a, ATCC 9277

Mutagen: UV

Depositor: RWB

Linkage Group: IVR

Mating Type: a

Species Number: 10

Genetic Background: M

Opposite Mating Type: 2982

ref1: Barratt et al 1954 Adv Genet 6:1-93, https://doi.org/10.1016/s0065-2660(08)60127-3

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
chol-1	IVR. Linked to ad-6 (1%). probably to the right (633). (482) Requires choline (470). Also uses mono- or dimethylaminoethanol (468) (Fig. 12). Deficient in S-adenosylmethionine:phosphatidylethanolamine-N-methyl transferase (222, 924). Abnormal colonial morphology on limiting choline (222). Colonies from single conidia on minimal agar medium resemble inhibited A/a duplications, with swollen hyphae and darkening in the presence of phenylalanine plus tyrosine (D.D. Perkins, unpublished data). Abnormal phospholipid composition on limited concentrations of supplement (501). Best scored late on minimal medium. Grows slightly and then stops (D.D. Perkins, unpublished data). Used to study inhibition of cytochrome-mediated respiration and of conidiation when lecithin is depleted by choline starvation (534, 535). Initial allele called 34486.	IVR	B