FGSC #463

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Reporting Genes: ad-4 leu-1

Species: crassa

Allele: Y112M160(F4) 33757

Alternate Strain Number: 74-YU590-3a(

Depositor: MEC

Linkage Group: IIIR IIIR

Mating Type: a

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ad-4	IIIR. Right of met-8 (1 to 4%) and com (0 to 5%). Left of leu-1 (1 to 3%) (219, 815). (482) Requires adenine. Cannot use hypoxanthine or inosine (661). Growth on adenine (at least for strains carrying allele 44206) is improved by the addition of histidine and still more by histidine plus methionine (661). Structural gene for adenylosuccinase, which controls two reactions in adenine synthesis (393, 1158). (See Fig. 8.) Accumulates a small amount of purple pigment when adenine is limiting (682). Used for the first demonstration of complementation between alleles in vivo (393) (simultaneous with independent demonstration in am) and in vitro (1157). Enzyme in revertants (1158). Used to study purine transport (787). Strains carrying alleles 44206 and 44415 are heat sensitive (34 C versus 25 C) (482, 682) and are osmotic remediable at 30°C (636); enzyme synthesized at 30°C by heat-sensitive strains has altered properties (636). Called complementation group F.	IIIR	B
leu-1	IIIR. Between ad-4 (1 to 5%) and his-7 (8%) (219, 578, 815, 1052). (868)Requires leucine (867, 868). Lacks beta-isopropylmalate dehydrogenase (426) (Fig. 15). Accumulates alpha-isopropylmalate and beta-isopropylmalate (427). Synthesis of the enzyme also requires the function of regulatory gene leu-3+ and the presence of alpha-isopropylmalate, which acts as inducer (427). Resistant to aminotriazole (D. D. Perkins, unpublished data). Female sterile (O. M. Mylyk, personal communication). Used to study reversion and competition in heterokaryons (901).	IIIR	B