FGSC #422

Add to Cart

Reporting Genes: En(pdx-1);pdx-1

Species: crassa

Allele: K30;44602p

Alternate Strain Number: 1293

Depositor: DDP

Linkage Group: IL;IVR

Mating Type: A

Species Number: 10

ref1: P.G. Martin Thesis (Genetics Dept., Adelaide, Aus)

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
pdx-1	IVR. Right of pyr-1 (<1 to 10%). Left of T(S1229) and pt (2%) (40, 55, 692, 808). (482) Uses pyridoxine, pyridoxal, or pyridoxamine (843, 845, 846). Shows intralocus complementation (845, 846) and recombination (848), Provided the first proven example of gene conversion (686). Scoring is sharpened by addition of 100 mg of desoxypyridoxine per liter (845). Several alleles (called pdxp: e.g., 44602) are pH sensitive and can grow without pyridoxine on medium containing ammonium ions at a pH above 6 (1029). Conidia are subject to death by unbalanced growth on minimal medium (1033). A yellow pigment is excreted under certain conditions by the pdx-1;En(pdx) double mutant; see En(pdx). Allele 44204 originally called pdx-2 (see reference 848).	IVR	B
En(pdx-1)	IL. Linked to mat (5%), probably to the left. The En(pdx); pdx-1 double mutant excretes yellow pigment when grown on Vogel's medium or Westergaard and Mitchell's mediumsupplemented with suboptimal pyridoxine plus ammonium sulfate at 5 mg/ml, but not at 1 mg/ml. Pigment is not produced by either single mutant. The addition of a nonlimiting concentration of pyridoxine inhibits production of the pigment on both media.	IL	B