FGSC #398

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Reporting Genes: cot-1 his-4

Species: crassa

Allele: C102(t) C141

Alternate Strain Number: C102,C141R1A

Depositor: MBM

Linkage Group: IVR IVR

Mating Type: A

Species Number: 10

ref1: Mitchell 1954 PNAS 40:436-440 M121, https://doi.org/10.1073/pnas.40.6.436

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
his-4	IVR. Between cot-1 (1 to 4%) and met-5 (4%) (812). (434)Requires histidine (434). Accumulates L-histidinol phosphate. Lacks histidinol phosphate phosphatase (24, 25) (Fig. 14). Allele P143h is heat sensitive (his+ at 25 C); C141 is not (815). Both are leaky (726).	IVR	B
cot-1	IVR. Between pan-1 (2%) and his-4 (1 to 6%) (692, 812, 816). Extremely colonial at 34°C, but completely normal growth, morphology, and fertility at 25°C and below. Linear growth is maximum at 24°C (374). Becomes colonial at 32°C; colonies from ascospores or conidia are viable and continue to grow slowly with dense branching, but do not conidiate. They quickly resume normal growth when shifted to a permissive temperature (692, 1068). Recessive in duplications (808); apparent dominance in heterokaryons (374) may have resulted from a shift in nuclear ratios. Used in studies of septation and branching (202), growth-inhibiting mucopolysaccharide (878, 879), and sulfate transport (641). Cell wall analysis (374). Growth is stimulated by lysine or arginine (0.1 mM) on glucose media at high temperatures (615). Because of high viability and tightly restricted growth at restrictive temperatures and normality at 25°C, cot-1 mutants have valuable technical applications. For example, crosses homozygous for cot-1 have been used in combination with sorbose for experiments with rec genes, where high-density ascospore platings are required for precise quantitative analysis of intralocus recombination (e.g., references 165, 997, and 1070). In another application, when shifted up after initial growth at the permissive low temperature, cot-1hyphae assume a "bottle brush" appearance with small side branches (692). This has been used to select uvs mutants by subsurface survival on UV-irradiated plates containing p-aminobenzoic acid (938; D.E.A. Catcheside, personal communication). cot-1 conidia or ascospores from cot-1 x cot-1crosses are used for replication in a protocol involving transfer by filter paper (615). For suppressors of cot-1, see gul.	IVR	B