FGSC #391

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Reporting Genes: col-4 pyr-2

Species: crassa

Allele: 70007R 38502

Alternate Strain Number: 38502,70007-

Depositor: MBM

Linkage Group: IVR IVR

Mating Type: A

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
col-4	IVR. Between met-1 (4%) and arg-2 (<1 to 2%) (692, 876, 991). (695) Spreading colonial morphology, forming dense balls of conidia high in slants (47). Probably dominant in heterozygous duplications from T(S1229) (E.G. Barry, personal communication). Cell wall-autolyzing enzyme (631). Reduced amount of cell wall peptides (1165). Used in combination with pe fl to produce microconidiating colonial growth suitable for reversion experiments (386). Called spco-1 (382); called c (386).	IVR	B
pyr-2	IVR. Right of nit-3 (2 to 9%). Left of mat (3%) and the T(NM152) right breakpoint (633, 812, 1000, PB). (482)Requires uracil or other pyrimidine. Lacks orotidine 5'-monophosphate pyrophosphorylase activity (133, 134) (Fig. 20). Needs medium containing >0.5 mg of uracil per ml for optimal growth. Allele 38502 is leaky. Pyrimidine biosynthetic pathway, showing sites of gene action (134, 456, 841, 1140). Carbamyl phosphate for arginine synthesis is made as a separate pool by a different enzyme system (see arg-2, arg-3, Fig. 10). Interchange between the two pools occurs only in certain mutant combinations. ATP, Adenosine 5'-monophosphate.	IVR	B