FGSC #358

Reporting Genes: nit-3

Species: crassa

Allele: 14789

Alternate Strain Number: 14789R4

Mutagen: X

Depositor: MBM

Linkage Group: IVR

Mating Type: a

Species Number: 10

Genetic Background: M

Opposite Mating Type: 3009

ref1: Houlahan et al. Genetics 34:493-507 1949 H127, https://www.genetics.org/content/34/5/493.long

Genes

Locus	Cultural Requirements	Link Group	Type
nit-3	IVR. Between met-5 (15%) and pyr-2 (2 to 9%) (1000, PB). (453) Cannot use nitrate as a nitrogen source, but uses nitrite, ammonia, hypoxanthine, or amino acids (28, 999). Structural gene for NADPH nitrate reductase (28) (Fig. 19). Allele 14789 apparently codes for an altered enzyme that cannot catalyze the whole electron transport sequence from NADPH to N03-, but can catalyze the terminal portion of this sequence, providing that a suitable electron donor (reduced viologen dye) is provided (999). See reference 198 for a model of interaction of nit-1 and nit-3 gene products. For regulation, see references 226, 999, and 1000. The nit-3+ gene has been cloned and is expressed in Escherichia coli (989).	IVR	B

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