FGSC #340

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Reporting Genes: leu-5

Species: crassa

Allele: 45208(t)

Alternate Strain Number: 45208T9C, ATCC 32314

Mutagen: UV

Depositor: MBM

Linkage Group: VR

Mating Type: a

Species Number: 10

Genetic Background: M

Opposite Mating Type: 939

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
leu-5	VR, Between cyh-2 (1%) and sp (3 to 9%) (818, 839, PB). (812)Strains carrying the only auxotrophic allele, 45208t. have a partial leucine requirement at low temperatures and a tighter leucine requirement at 34°C and stop growth at 37 to 39 C, regardless of leucine supplementation (290, 839). Altered leucyl-tRNA synthetase (839). Apparently a gene complex consisting of structural genes for cytoplasmic leucyl-tRNA synthetase and a separate mitochondrial leucyl-tRNA synthetase. Mutations mapping in the leu-5 region can affect either enzyme separately or both simultaneously (69, 431). In the [cni-3] mitochondrial mutant, mitochondrial tRNA synthetase is greatly increased, whereas the cytoplasmic enzyme is unchanged (430). Allele 45208t causes alterations in unrelated enzymes, apparently via mistranslation (607, 839). Used to study the hypothesis that senescence is due to faulty protein synthesis (607). Assembly of glycerolkinase and glycerol-3-phosphate dehydrogenase into inner mitochondrial membrane is not impaired in leu-5 strains (213). Poor recovery from ascospores at 34°C (complex complete medium); best germinated at 25°C. Allele 45208t is somewhat unstable (431).	VR	B