FGSC A1148

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Reporting Genes: pyrG89; nkuB::A. fumigatus riboB pyroA4 nkuA::argB; riboB2

Species: nidulans

Alternate Strain Number: TN12, LO1414

Depositor: BRO

Species Number: 1

ref1: https://www.genetics.org/content/172/3/1557.long

ref2: https://doi.org/10.1534/genetics.105.052563

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
pyrG89	Optimal supplementation is 2.5 mg/ml (10 mM) each of uridine + uracil. pyrG89is cold sensitive and sectors	IL	A
pyroA4	Responds to pyridoxine	IVR	A
riboB2	Requires riboflavin. Confers self-sterility	VIIIR	A
nkuA::argB	Arginine requirement. The nkuA gene was deleted in KJ12 by replacing it with argB. This was crossed to A770. TN02A7 is a segregant of this cross. Deletion of the nkuA (the A. nidulans homolog of human Ku70 gene) reduces the frequency of non-homologous integration of transforming DNA and greatly facilitates gene targeting. NkuA deleted strains show no obvious growth defect and are not sensitive to a variety of DNA damaging drugs.		A
nkuB::A. fumigatus riboB	Riboflavin requirement. The nkuA gene was deleted in KJ12 by replacing it with argB. This was crossed to A770. TN02A7 is a segregant of this cross. NkuB was deleted in TN02A7 by replacing it with the A. fumigatus riboB gene. NkuA and nkuB form a heterodimer important for non-homologous end joining of DNA. Deletion of nkuA greatly reduces the frequency of insertion of transforming DNA by non-homologous integration and, thus, greatly facilitates gene targeting by homologous recombination. Deletion of nkuB (by replacement with the A. fumigatus riboB gene) in an nkuA deletion background does not cause any improvement in gene targeting relative to an nkuA deletion. NkuA/nkuB double deletion strains show no obvious difference in growth or sensitivity to mutagens when compared to nkuA single deletion ro wild-type strains.		A