FGSC A1145

Reporting Genes: pyrG89; pyroA4; nkuA::argB; riboB2

Species: nidulans

Alternate Strain Number: TN02A7, LO1362

Depositor: BRO

Species Number: 1

ref1: https://www.genetics.org/content/172/3/1557.long

ref2: https://doi.org/10.1534/genetics.105.052563

Genes

Locus	Cultural Requirements	Link Group	Type
pyrG89	Optimal supplementation is 2.5 mg/ml (10 mM) each of uridine + uracil. pyrG89is cold sensitive and sectors	IL	A
nkuA::argB	Arginine requirement. The nkuA gene was deleted in KJ12 by replacing it with argB. This was crossed to A770. TN02A7 is a segregant of this cross. Deletion of the nkuA (the A. nidulans homolog of human Ku70 gene) reduces the frequency of non-homologous integration of transforming DNA and greatly facilitates gene targeting. NkuA deleted strains show no obvious growth defect and are not sensitive to a variety of DNA damaging drugs.		A
pyroA4	Responds to pyridoxine	IVR	A
riboB2	Requires riboflavin. Confers self-sterility	VIIIR	A

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