FGSC #263

Add to Cart

Reporting Genes: al-2 cnr

Species: crassa

Allele: 15300 no#(s)

Alternate Strain Number: 1260?

Depositor: DDP

Linkage Group: IR

Mating Type: a

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
cnr	IR. Linked to hom (1%) and nic-1 (11%), probably between them (812). (1061) Resistance to canavanine (480, 1061, 1062) is due to a constitutive enzyme that cleaves L-canavanine to hydroxyguanidine plus a compound which can be converted to L-homoserine (617). Resistance is altered secondarily by modifiers that affect rate of uptake (617). Many laboratory strains are resistant, but a few are sensitive (790). Best scored on 0.2 mg of L-canavanine H2SO4 per ml (autoclaved in the medium) at 2 days, 34°C (812; D.D. Perkins, unpublished data). Sensitive strain used to select resistant mutants defective in basic amino acid transport (889, 913, 1152); see pmb. Called can.	IR	B
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B