FGSC #231

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Reporting Genes: al-2 sc lys-1

Species: crassa

Allele: 15300;5801;33933

Alternate Strain Number: 9071

Depositor: DDP

Linkage Group: IR;IIIR;VC

Mating Type: a

Species Number: 10

Opposite Mating Type: 230

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
sc	IIIR. Between the centromere and ser-1 (504). Linked to thi-4 (1/280) and spg(0 + + /179); right of acr-2 (3 to 6%) (498, 814). Irregular flat spreading growth with knobby protrusions but no conidia. Mycelium usually appears yellowish rather than orange. Female fertile. Homozygous sc x sc crosses give nonlinear asci (827, 828, 1011). Cell wall analysis (132). Reduced amount of cell wall peptides (1165). Allele R2503 called col-14; allele R2386 called smco-2 (382). For modifier, see mod(sc).	IIIR	B
lys-1	V. Right of caf-1 (4 to 14%). Left of cyt-9 (5%) and at (1 to 20%) (817; K.S. Hsu, personal communication; PB). (403)Uses lysine, alpha-aminoadipic acid, or epsilon-hydroxynorleucine (alpha-amino-epsilon-hydroxycaproic acid) (399, 400, 684, 1087). Accumulates homocitrate on limiting lysine concentrations (464) (Fig. 16). Fine structure and complementation between alleles (8). Initial allele: 33933.	V	B
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B