FGSC #206

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Reporting Genes: his-3 pt

Species: crassa

Allele: C1710;S4342

Alternate Strain Number: 1X013

Depositor: DDP

Linkage Group: IR;IVR

Mating Type: a

Species Number: 10

ref1: Barratt et al 1954 Adv Genet 6:1-93, https://doi.org/10.1016/S0065-2660(08)60127-3

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
his-3	IR. Right of met-10 (R.L. Metzenberg, personal communication). Left of cog (1 to 3%) (172,174), ure-4 (1%) (78), and ad-3A (1%) (271). (434)Requires histidine (434). Complex gene coding for histidinol dehydrogenase, phosphoribosyladenosine 5'-triphosphate-pyrophosphohydrolase, and phosphoribosyl-adenosine 5'-monophosphate-cyclohydrolase (16, 673) (Fig. 14). All three activities appear to be catalyzed by a single protein (673). Strains carrying different individual alleles may lack only the early reaction(s) or only histidinol dehydrogenase, or both. Those that lack only histidinol dehydrogenase accumulate histidinol (16, 162, 1123). Mutants produce cross-reacting material (220). Used to study intralocus complementation and recombination (15, 16, 27, 162, 164, 171, 172, 1121, 1122, 1124). Intralocus recombination is regulated by cog and by rec-2 (27, 171); it is not affected by rec-1 (172). Translocation T(IR;VII)TM429, with one breakpoint in his-3, has been used to show that cog is cis-acting (171). Initial alleles: C140 and T1710 (= C1710).	IR	B
pt	IVR. Right of T(S1229) and pdx-1 (2%). Left of col-4(2%) (40, 55, 808). (201) Original S4342 strain contained linked but separable insertional translocation T(S4342) (808), the presence of which should not change conclusions regarding gene order given in reference 40. Requires phenylalanine plus tyrosine (201). Lacks chorismate mutase (40, 316) (Fig. 11). Evidently the structural gene; strains carrying allele NS1 have thermolabile chorismate mutase (D.E.A. Catcheside, personal communication). NS1 strains are temperature sensitive, growing on minimal medium at 25 C, where they are readily scorable by blue fluorescence under long-wave UV and by browning of medium of aging cultures (1035). Inhibited by complex complete medium.	IVR	B