FGSC #4771

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Reporting Genes: Not available rg-1, his-3, nic-2

Species: crassa

Allele: B53, 74A-4152-M9, 43002

Alternate Strain Number: 53-OR6-3a

Depositor: FJD

Linkage Group: IR,R,R

Mating Type: a

Species Number: 10

Opposite Mating Type: 4770

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
his-3	IR. Right of met-10 (R.L. Metzenberg, personal communication). Left of cog (1 to 3%) (172,174), ure-4 (1%) (78), and ad-3A (1%) (271). (434)Requires histidine (434). Complex gene coding for histidinol dehydrogenase, phosphoribosyladenosine 5'-triphosphate-pyrophosphohydrolase, and phosphoribosyl-adenosine 5'-monophosphate-cyclohydrolase (16, 673) (Fig. 14). All three activities appear to be catalyzed by a single protein (673). Strains carrying different individual alleles may lack only the early reaction(s) or only histidinol dehydrogenase, or both. Those that lack only histidinol dehydrogenase accumulate histidinol (16, 162, 1123). Mutants produce cross-reacting material (220). Used to study intralocus complementation and recombination (15, 16, 27, 162, 164, 171, 172, 1121, 1122, 1124). Intralocus recombination is regulated by cog and by rec-2 (27, 171); it is not affected by rec-1 (172). Translocation T(IR;VII)TM429, with one breakpoint in his-3, has been used to show that cog is cis-acting (171). Initial alleles: C140 and T1710 (= C1710).	IR	B
nic-2	IR. Between ad-3B (4%) and ace-7 (4 to 7%) (271, 578). (482) Grows on nicotinic acid, nicotinamide, or high concentrations of quinolinic acid (97, 1168). Cannot use kynurenine, hydroxykynurenine, or hydroxyanthranilic acid (96, 1168). Accumulates 3-hydroxyanthranilic acid (96) (Fig. 18). Aging cultures accumulate red-brown pigment in the medium. Used to study intralocus recombination (908). Translocations T(4540) and T(S1325)are inseparable from nic-2 (808, 908, 911).	IR	B
rg-1	IR. Right of T(AR173); hence, of his-2. Left of lys-4 (1 to 7%) (271, 789, 810).Spreading dense colonial growth with poor conidiation (789). Increased hyphal branching; bumpy mycelial surface. Altered phosphoglucomutase (isozyme I). Accumulates glucose-1-phosphate (117). Cell wall composition (132). Normal levels of NADPH (110) and linolenic acid (115). Photograph (112). The double mutant rg cr grows as small discrete conidiating colonies suitable for velvet replication (634). For examples of applications, see references 932 and 1020. Unlike sn cr, which it resembles phenotypically, the double mutant rg cr is not homozygous fertile. Allele R2357 was formerly called er: erupt (see reference 382). Allele S4357 was formerly called col-7 (see reference 675).	IR	B