FGSC #9529

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Reporting Genes: qde-1;Sad-1;fl

Species: crassa

Allele: qde-1::Bml^R; Sad-1^Delta ::hygR; fl^P

Alternate Strain Number: 11-23

Depositor: RLM

Linkage Group: VII;IL

Mating Type: a

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
fl	IIR. Between ace-1 (5 to 11%) and trp-3 (3%) (816, PB). (613)No macroconidia (609). Highly fertile (612). Used routinely as the female parent in tests for chromosome rearrangements and for mating type (e.g., reference 801). The flsingle mutant produces few microconidia when dry; when wetted, sufficient microconidia are produced to have been used in early irradiation and mutation studies (614, 915); large numbers can be obtained under certain conditions; see reference 893. pe fl (46, 700) and fl;dn (806) double mutants produce abundant microconidia; the latter combination is highly fertile when homozygous. Photograph of microconidial formation (774); see also reference 893. Nuclear numbers in microconidia (46, 64, 478). Wall analysis (207). Immunoelectrophoretic pattern (784). Paradoxical high alcoholic glycolysis on nitrate medium (80). Deficiency of isocitrate lyase on acetate medium; see citations in reference 1088. When fl A and fl a strains are inoculated separately on crossing medium in plates, a double line of perithecia forms where they meet, similar to that accompanying barrage in Podospora (410, 414). fl ascospores from certain fl x fl+ crosses often germinate spontaneously (1127; N. B. Raju, personal communication). Allele C-1835 was called acon (717, 812).	IIR	B
sad-1	Suppresses several classical ascus-dominant mutants. Also, suppresses the near-absolute barren phenotype of crosses between wild type and a variety of strains in which a segment of DNA is duplicated. Finally, interspecies crosses within the genus Neurospora that are normally almost completely infertile become much fertile if the N. crassa parent is a Sad-1 mutant, suggesting that MSUD due to numerous small mispairings play a role in reproductive isolation of these species. sad-1+ encodes a RNA-directed RNA polymerase.		B
qde-1	qde-1 is Barren in homozygous crosses. It can be conveniently scored on lawns of qde-1::Bml^R; Sad-1^delta::hygR;fl^P (FGSC 9528 and 9529). Fertile in crosses with wild type (point mutation)	VII	B