FGSC #9453

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Reporting Genes: spco-4;gna-1

Species: crassa

Allele: R2367, ?

Alternate Strain Number: OG88

Depositor: OG

Linkage Group: VIIL, IIIR

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
spco-4	VIIL. Linked to do (<1%) and nic-3 (1%, probably to the left) (816). Fine hyphae (382). Initially aconidial. Capable of conidiating on the surface of complete medium (D.D. Perkins, unpublished data). Hyphae extend faster within agar medium than on the surface, resulting in a dense hemispherical colony, most of which is embedded (1085).	VIIL	B
gna-1	IIIR. Linked to ro-2 (0/17). Encodes guanine nucleotide-binding protein alpha-1-subunit. The predicted amino acid sequence shows significant homology to the Gi family found in higher organisms. The null mutant shows female infertility, osmotic sensitivity, reduced hyphal growth rate, and defective macroconidiation, has decreased intracellular cAMP levels, and is more resistant to some environmental stresses than wild type. Mutants that are predicted to maintain an activated state of GNA-1 have increased intracellular cAMP levels, overproduce aerial hyphae, and are more sensitive to environmental stresses than wild type. Null-mutant extracts have reduced Mg^2+ -dependent adenylyl cyclase and cAMP phosphodiesterase activity. Mg^2+ -dependent adenylyl cyclase activity in wild-type extracts can be inhibited by using antibody against GNA-1.	IIIR	B