FGSC #9450

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Reporting Genes: gna-1 col-15

Species: crassa

Allele: ? , R2531

Alternate Strain Number: OG85

Depositor: OG

Linkage Group: IIIR

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group
gna-1	IIIR. Linked to ro-2 (0/17). Encodes guanine nucleotide-binding protein alpha-1-subunit. The predicted amino acid sequence shows significant homology to the Gi family found in higher organisms. The null mutant shows female infertility, osmotic sensitivity, reduced hyphal growth rate, and defective macroconidiation, has decreased intracellular cAMP levels, and is more resistant to some environmental stresses than wild type. Mutants that are predicted to maintain an activated state of GNA-1 have increased intracellular cAMP levels, overproduce aerial hyphae, and are more sensitive to environmental stresses than wild type. Null-mutant extracts have reduced Mg^2+ -dependent adenylyl cyclase and cAMP phosphodiesterase activity. Mg^2+ -dependent adenylyl cyclase activity in wild-type extracts can be inhibited by using antibody against GNA-1.	IIIR
col-15	IIIR. Putative vel allele (0/26) (PB). Linked to tyr-1 (5%) and col-13(0/181) (382). Resembles col-13 and vel. Occasionally forms puffs of aerial conidia at tops of slants; these are not due to reversion (PB). Reported to complement col-13in heterokaryons (382), but this may have been due to misinterpretation of erratic conidiation.	IIIR