FGSC A1122

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Reporting Genes: nimG10, chaA, lac, nic

Species: nidulans

Alternate Strain Number: SWJ 003

Depositor: SGWK

Species Number: 1

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
lac	Multigenic basis. No major gene identified. Strains designated lac showed poor growth on lactose. Lactase (beta-D-galactosidase) properties were unaltered; levels were normal when grown on sucrose and depressed on lactose (584, 585). These strains differ from wild type at several loci, each with a small and additive effect on lactose utilization; e.g., three component genes from "lac-" strain 31389 x wild type were shown to be unlinked and were designated n-lac-1, pow(n-lac-2) (powdery conidia), and floc(nlac-3) (flocculent morphology). These genes are not specific for lactose utilization but also result in an altered adaptation response to other carbon sources and in other pleiotropic effects. Thus, probably no identified locus qualifies to be designated lac. (357) The failure to find a single gene mutant unable to use lactose is ascribed to the fact that Neurospora has two beta-galactosidases (605 and references therein).		B
nic	nic mutants are preferably supplemented with nicotinamide rather than nicotinic acid at most pH values because of permeability (97). nt mutants are best treated as nicmutants for purposes of growth and scoring. For good recovery of some nicmutants from crosses, crossing media should be supplemented with nicotinamide at levels higher (10X) than those required for growth, even when the protoperithecial parent is nic+ (789; P. St. Lawrence, personal communication). See Fig. 18 for the biosynthetic pathway. For regulation, see references 111, 371, 604, and 926.		B
nimG10	(II). Never in mitosis	(II)	A
chaA	VIII. Charteuse colored conidia	VIII	A