FGSC #170

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Reporting Genes: pdx-1 pan-1 pyr-2

Species: crassa

Allele: 37803 5531 38502

Alternate Strain Number: 4016

Depositor: DDP

Linkage Group: IVR IVR IVR

Mating Type: a

Species Number: 10

Opposite Mating Type: 161

ref1: Maling 1959 Genetics 44:1215-1220, https://www.genetics.org/content/44/6/1215.long

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
pan-1	IVR. Between ad-6 (1 to 2%) and cot-1 (2 to 3%) (633, 692, PB). (482). cel, col-1, int, pho-3, and thi-5 all appear to be closely linked in this crowded region. Requires intact pantothenic acid for growth under standard conditions. Able to synthesize both precursors, beta-alanine and pantoyl lactone (1058). Ability to synthesize pantothenic acid from beta-alanine plus pantoyl lactone is demonstrable in vitro but not in vivo unless cultures are aerated (1111, 1113, 1114). Unlike pan-2, pan-1 has no effect on ascospore ripening in heterozygous crosses. Called group A. For alleles see reference 138.	IVR	B
pdx-1	IVR. Right of pyr-1 (<1 to 10%). Left of T(S1229) and pt (2%) (40, 55, 692, 808). (482) Uses pyridoxine, pyridoxal, or pyridoxamine (843, 845, 846). Shows intralocus complementation (845, 846) and recombination (848), Provided the first proven example of gene conversion (686). Scoring is sharpened by addition of 100 mg of desoxypyridoxine per liter (845). Several alleles (called pdxp: e.g., 44602) are pH sensitive and can grow without pyridoxine on medium containing ammonium ions at a pH above 6 (1029). Conidia are subject to death by unbalanced growth on minimal medium (1033). A yellow pigment is excreted under certain conditions by the pdx-1;En(pdx) double mutant; see En(pdx). Allele 44204 originally called pdx-2 (see reference 848).	IVR	B
pyr-2	IVR. Right of nit-3 (2 to 9%). Left of mat (3%) and the T(NM152) right breakpoint (633, 812, 1000, PB). (482)Requires uracil or other pyrimidine. Lacks orotidine 5'-monophosphate pyrophosphorylase activity (133, 134) (Fig. 20). Needs medium containing >0.5 mg of uracil per ml for optimal growth. Allele 38502 is leaky. Pyrimidine biosynthetic pathway, showing sites of gene action (134, 456, 841, 1140). Carbamyl phosphate for arginine synthesis is made as a separate pool by a different enzyme system (see arg-2, arg-3, Fig. 10). Interchange between the two pools occurs only in certain mutant combinations. ATP, Adenosine 5'-monophosphate.	IVR	B