FGSC #137

Reporting Genes: pt ylo-1

Species: crassa

Allele: NS1(t);Y30539y

Alternate Strain Number: 4048

Depositor: DDP

Linkage Group: IVR;VIIL

Mating Type: A

Species Number: 10

Genetic Background: M

Opposite Mating Type: 10

ref1: Perkins, '60 mimeo note, suppl 1959 Genetics 44:1185-1208 P36

Genes

Locus	Cultural Requirements	Link Group	Type
ylo-1	VIL. Between cys-1 (8%) and ad-1 (6%). Probably right of Bml (2%) (1012, PB). (381). Yellow carotenoids (381). Affects synthesis of neurosporaxanthin (4'-apo-beta'-caroten-4'-oic acid); citations in reference 398. Lesion probably involves the conversion of lycopene to 3,4-dehydrolycopene or the conversion of either torulene or gamma-carotene to neurosporaxanthin (398 and references therein) (Fig. 9). Resembles the orange wild type in young cultures, but color differences become clear with age. Expressed in both conidia and mycelia. Undefined modifiers affect intensity. Fails to complement with many of the al-1 and al-2 albino strains (R.E. Subden, personal communication).	VIL	B
pt	IVR. Right of T(S1229) and pdx-1 (2%). Left of col-4(2%) (40, 55, 808). (201) Original S4342 strain contained linked but separable insertional translocation T(S4342) (808), the presence of which should not change conclusions regarding gene order given in reference 40. Requires phenylalanine plus tyrosine (201). Lacks chorismate mutase (40, 316) (Fig. 11). Evidently the structural gene; strains carrying allele NS1 have thermolabile chorismate mutase (D.E.A. Catcheside, personal communication). NS1 strains are temperature sensitive, growing on minimal medium at 25 C, where they are readily scorable by blue fluorescence under long-wave UV and by browning of medium of aging cultures (1035). Inhibited by complex complete medium.	IVR	B

Neurospora Crassa Wikipedia