FGSC #7053 Mating Types: a Species: Neurospora crassa
Genotype: fl;wc-1
Alleles: P;P829
Linkage Group(s): IIR;VIIR
Genetic Background: SL
Stock No. from Other Collection: 9271
Depositor of Strain: DDP
Strain of Opposite Mating Type: 7052
Markers
Lesion: fl fluffy
Lesion Information for Marker:
      Linkage Group of fl: IIR
      Markers Left of fl: ace-1 (5 to 11%)
      Markers Right offl: trp-3 (3%)
Marker description or requirements:
      IIR. Between ace-1 (5 to 11%) and trp-3 (3%) (816, PB). (613)No macroconidia (609). Highly fertile (612). Used routinely as the female parent in tests for chromosome rearrangements and for mating type (e.g., reference 801). The flsingle mutant produces few microconidia when dry; when wetted, sufficient microconidia are produced to have been used in early irradiation and mutation studies (614, 915); large numbers can be obtained under certain conditions; see reference 893. pe fl (46, 700) and fl;dn (806) double mutants produce abundant microconidia; the latter combination is highly fertile when homozygous. Photograph of microconidial formation (774); see also reference 893. Nuclear numbers in microconidia (46, 64, 478). Wall analysis (207). Immunoelectrophoretic pattern (784). Paradoxical high alcoholic glycolysis on nitrate medium (80). Deficiency of isocitrate lyase on acetate medium; see citations in reference 1088. When fl A and fl a strains are inoculated separately on crossing medium in plates, a double line of perithecia forms where they meet, similar to that accompanying barrage in Podospora (410, 414). fl ascospores from certain fl x fl+ crosses often germinate spontaneously (1127; N. B. Raju, personal communication). Allele C-1835 was called acon (717, 812).
Reference for: fl: 46. Barratt, R. W. and L. Garnjobst. 1949. Genetics of a colonial microconidiating mutant strain of N. crassa. Genetics 34:351-369.
Reference for: fl: 64. Baylis, J. R. , Jr. , and A. G. DeBusk. 1967. Estimation of the frequency of multinucleate conidia in microconidiating strains. NeurosporaNewsl. 11:9.
Reference for: fl: 80. Bernardini, D. , and G. Turian. 1978. Recherches sur la differenciation conidienne de N. crassa. VII. Regulation alcooligene et capacite deconidiation (souche sauvage et mutant "fluffy"). Ann Microbiol. (Paris) 129B:551-559.
Reference for: fl: 207. Coniordos, N. , and G. Turian. 1973. Recherches sur la differenciation conidienne de N. crassa. IV. Modifications chimio-structurales de laparoi chez le type sauvage et chez deux mutants aconidiens. Ann. Microbiol. (Paris) 124A:5-28.
Reference for: fl: 410. Griffiths, A. J. F. 1979. A Neurospora experiment for an introductory biology course. Neurospora Newsl. 26:12.
Reference for: fl: 414. Griffiths, A. J. F. , and A. Rieck. 1981. Perithecial distribution patterns in standard and variant strains of Neurospora crassa. Can. J. Bot. 59:2610-2617,.
Reference for: fl: 478. Horowitz, N. H. , and H. Macleod. 1960. The DNA content of Neurospora nuclei. Microb. Genet. Bull. 17:6-7.
Reference for: fl: 609. Lindegren, C. C. 1933. The genetics of Neurospora. III. Pure bred stocks and crossing-over in N. crassa. Bull. Torrey Bot. Club 60:133-154.
Reference for: fl: 612. Lindegren, C. C. , V. Beanfield, and R. Barber. 1939. Increasing the fertility of Neurospora by selective inbreeding. Bot. Gaz. 100:592-599.
Reference for: fl: 613. Lindegren, C. C. , and G. Lindegren. 1939. Non-random crossing-over in the second chromosome of Neurospora crassa. Genetics 24:1-7.
Reference for: fl: 614. Lindegren, C. C. , and G. Lindegren. 1941. X-ray and ultraviolet induced mutations in Neurospora. I. X-ray mutations. J. Hered. 32:404-412.
Reference for: fl: 700. Munkres, K. D. 1977. Selection of improved microconidial strains of Neurospora crassa. Neurospora Newsl. 24:9-10.
Reference for: fl: 717. Murray, J. C. , and A. M. Srb. 1962. The morphology and genetics of wild-type and seven morphological mutant strains of Neurosporacrassa. Can. J. Bot. 40:337-350.
Reference for: fl: 774. Oulevey-Matikian, N. , and G. Turian. 1968. Contrle metabolique et aspects ultrastructuraux de la conidiation (macro-microconidies) deNeurospora crassa. Arch. Mikrobiol. 60:35-58.
Reference for: fl: 784. Peduzzi, R. , and G. Turian. 1972. Recherches sur la differenciation conidienne de Neurospora crassa. III. Activitemalico-deshydrogenasique de structures antigeniques et ses relations avec la competence conidienne. Ann. Inst. Pasteur 122:1081-1097.
Reference for: fl: 801. Perkins, D. D. 1974. The manifestation of chromosome rearrangements in unordered asci of Neurospora. Genetics 77:459-489.
Reference for: fl: 806. Perkins, D. D. 1979. A new, highly fertile microconidiating combination, dingy, fluffy. Neurospora Newsl. 26:9.
Reference for: fl: 812. Perkins, D. D. , M. Glassey, and B. A. Bloom. 1962. New data on markers and rearrangements in Neurospora. Can. J. Genet. Cytol. 4:187-205.
Reference for: fl: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.
Reference for: fl: 915. Sansome, E. R. , M. Demerec, and A. Hollaender. 1945. Quantitative irradiation experiments with Neurospora crassa. I. Experiments withX-rays. Am. J. Bot. 32:218-226.
Reference for: fl: 1127. Weijer, J. 1954. A genetical investigation into the td-locus of Neurospora crassa. Genetica 28:173-252.
Lesion: wc-1 white collar-1
Lesion Information for Marker:
      Linkage Group of wc-1: VIIR
      Markers Left of wc-1: met-9 (1 to 4%)
      Markers Right ofwc-1: un-10 (7%) and for (6%)
Marker description or requirements:
      VIIR. Right of met-9 (1 to 4%). Left of un-10 (7%) and for (6%) (724, 812, 816). Carotenoids absent from mycelia; conidia become pigmented with some delay. Named because nonconidiating rim at top of agar slant remains white. A double mutant with flor other nonconidiating mutant would be classed as albino. Regulatory mutants for photoinduced carotenogenesis via blue light receptor might be expected to have a similar phenotype (444, 445). A blue light treatment (given in vivo), which increases the activity of soluble and microsomal enzymes required for phytoene biosynthesis in the wild type, does not do so in the mutant wc-1 (445). Fails to show phototropism of perithecial beaks when used as the female (protoperithecial) parent, but not when used as the male (fertilizing) parent (R. W. Harding, personal communication). Useful genetic marker (725, 800). Scoring clearest at high temperatures (34 C).
Reference for: wc-1: 444. Harding, R. W. , and W. Shropshire, Jr. 1980. Photocontrol of carotenoid biosynthesis. Annu. Rev. Plant Physiol. 31:217-238.
Reference for: wc-1: 445. Harding, R. W. , and R. V. Turner. 1981. Photoregulation of the carotenoid biosynthetic pathway in albino and white collar mutants ofNeurospora crassa. Plant Physiol. 68:745-749.
Reference for: wc-1: 724. Murray, N. E. 1969. Reversal of polarized recombination of alleles in Neurospora as a function of their position. Genetics 61:67-77.
Reference for: wc-1: 725. Murray, N. E. 1970. Recombination events that span sites within neighbouring gene loci of Neurospora. Genet. Res. 15:109-121.
Reference for: wc-1: 800. Perkins, D. D. 1972. Linkage testers having markers near the centromere. Neurospora Newsl. 19:33.
Reference for: wc-1: 812. Perkins, D. D. , M. Glassey, and B. A. Bloom. 1962. New data on markers and rearrangements in Neurospora. Can. J. Genet. Cytol. 4:187-205.
Reference for: wc-1: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.

 

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6/11/12