FGSC #6427 Mating Types: a Species: Neurospora crassa
Genotype: mus-25 nic-3
Alleles: FK123 Y31881
Linkage Group(s): VIIR L
Stock No. from Other Collection: M2388
Depositor of Strain: EK
Strain of Opposite Mating Type: 6426
Reference: Kafer and Luk, FGN 35
Lesion: mus-25 mutagen sensitive-25
Lesion Information for Marker:
      Linkage Group of mus-25: VIIC
Marker description or requirements:
      VIIC. Between met-7(4%, 6%) and un-10. Cloned. Epistasis group Uvs-6. Homologous to yeast RAD54, a gene involved in homologous recombination with sequence conserved from fungi to humans. Two alleles, SA3 and FK123. Both are highly sensitive to histidine, moderately sensitive to MMS, MNNG, and gamma rays, and not sensitive to UV or mitomycin C. At least FK123 is slightly sensitive to bleomycin. Homozygous barren.
Lesion: nic-3 nicotinic acid-3
Lesion Information for Marker:
      Linkage Group of nic-3: VIIL
      Markers Left of nic-3: spco-4 (1%) and do (3%)
      Markers Right ofnic-3: thi-3 (9 to 27%) and csp-2 (16 to 22%)
Marker description or requirements:
      VIIL. Right of spco-4 (1%) and do (3%). Left of thi-3 (9 to 27%) and csp-2 (16 to 22%) (539, 812, 816, PB). (M.K. Allen, cited in references 718 and 789) Uses nicotinic acid, nicotinamide, 3-hydroxyanthranilic acid, 3-hydroxykynurenine, or high concentrations of quinolinic acid (96, 1168). Accumulates alpha-N-acetylkynurenine; blocked in conversion of kynurenine to 3-hydroxykynurenine (1168) (Fig. 18). Pyridine nucleotide levels (111). Pathway from tryptophan to nicotinic mononucleotide, showing sites of gene action (96, 100, 368, 1168). The enzymatic reactions between 3-hydroxyanthranilate and nicotinic mononucleotide have not been demonstrated directly in Neurospora.
Reference for: nic-3: 96. Bonner, D. M. 1948. The identification of a natural precursor of nicotinic acid. Proc. Natl. Acad. Sci. U. S. A. 34:5-9.
Reference for: nic-3: 100. Bonner, D. M. , and C. Yanofsky. 1949. Quinolinic acid accumulation in the conversion of 3-hydroxyanthranilic acid to niacin inNeurospora. Proc. Natl. Acad. Sci. U. S. A. 35:576- 581.
Reference for: nic-3: 111. Brody, S. 1972. Regulation of pyridine nucleotide levels and ratios in Neurospora crassa. J. Biol. Chem. 247:6013-6017.
Reference for: nic-3: 368. Gaertner, F. H. 1981. A response to a letter from David Perkins concerning the question of whether nt mutants involve tryptophanpyrrolase. Neurospora Newsl. 28:12.
Reference for: nic-3: 539. Kafer, E. , and E. Perlmutter. 1980. Isolation and genetic analysis of MMS-sensitive mus mutants of Neurospora. Can. J. Genet. Cytol. 22:535-552.
Reference for: nic-3: 718. Murray, N. E. 1960. The distribution of methionine loci in Neurospora crassa. Heredity 15:199-206.
Reference for: nic-3: 789. Perkins, D. D. 1959. New markers and multiple point linkage data in Neurospora. Genetics 44:1185-1208.
Reference for: nic-3: 812. Perkins, D. D. , M. Glassey, and B. A. Bloom. 1962. New data on markers and rearrangements in Neurospora. Can. J. Genet. Cytol. 4:187-205.
Reference for: nic-3: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.
Reference for: nic-3: 1168. Yanofsky, C. , and D. M. Bonner. 1950. Evidence for the participation of kynurenine as a normal intermediate in the biosynthesis of niacinin Neurospora. Proc. Natl. Acad. Sci. U. S. A. 36:167-176.


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