FGSC #5966 Mating Types: + Species: Fusarium verticillioides
Genotype: Fusarium verticillioides nit3
Alleles: FKMA962
Linkage Group(s): VCG A1
Stock No. from Other Collection: FKMA962
Depositor of Strain: JFL
Strain of Opposite Mating Type: 0
Reference: Klittich and Leslie 1988. Genetics 118:417-423
Lesion: nit-3 nitrate nonutilizer-3
Enzyme Name: NADPH nitrate reductase
Lesion Information for Marker:
      Linkage Group of nit-3: IVR
      Markers Left of nit-3: met-5 (15%)
      Markers Right ofnit-3: pyr-2 (2 to 9%)
Marker description or requirements:
      IVR. Between met-5 (15%) and pyr-2 (2 to 9%) (1000, PB). (453) Cannot use nitrate as a nitrogen source, but uses nitrite, ammonia, hypoxanthine, or amino acids (28, 999). Structural gene for NADPH nitrate reductase (28) (Fig. 19). Allele 14789 apparently codes for an altered enzyme that cannot catalyze the whole electron transport sequence from NADPH to N03-, but can catalyze the terminal portion of this sequence, providing that a suitable electron donor (reduced viologen dye) is provided (999). See reference 198 for a model of interaction of nit-1 and nit-3 gene products. For regulation, see references 226, 999, and 1000. The nit-3+ gene has been cloned and is expressed in Escherichia coli (989).
Reference for: nit-3: 28. Antoine, A. D. 1974. Purification and properties of the nitrate reductase isolated from N. crassa mutant nit-3. Kinetics, molecular weightdetermination, and cytochrome involvement. Biochemistry 13:2289-2294.
Reference for: nit-3: 453. Head, J. J. 1955. The reduction of nitrate to nitrite by Neurospora crassa:a physiological and genetical study. Ph. D. thesis, University ofCambridge. Abstr. Diss. Univ. Cambridge 1954-1955:21-22 (1957).
Reference for: nit-3: 989. Smarelli, J. , Jr. , and R. H. Garrett. 1982. Isolation of the Neurospora crassa nitrate reductase structural gene:evidence for its expression inEscherichia coli. Fed. Proc. 41:756 (Abstr.).
Reference for: nit-3: 999. Sorger, G. J. 1966. Nitrate reductase electron transport systems in mutant and in wild-type strains of Neurospora. Biochim. Biophys. Acta 118:484-494.
Reference for: nit-3: 1000. Sorger, G. J. , and N. H. Giles. 1965. Genetic control of nitrate reductase in Neurospora crassa. Genetics 52:777-788.


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