FGSC #5965 Mating Types: + Species: Fusarium verticillioides
Genotype: Fusarium moniliforme nit2
Alleles: FKMA961
Linkage Group(s): VCG A1
Stock No. from Other Collection: FKMA961
Depositor of Strain: JFL
Strain of Opposite Mating Type: 0
Reference: Klittich and Leslie 1988. Genetics 118:417-423
Lesion: nit-2 nitrate nonutilizer-2
Enzyme Name: regulatory
Lesion Information for Marker:
      Linkage Group of nit-2: IL
      Markers Left of nit-2: T(39311) left breakpoint and un-5(2%)
      Markers Right ofnit-2: In(OY323) and leu-3 (12 to 18%)
Marker description or requirements:
      IL. Right of the T(39311) left breakpoint and of un-5(2%). Left of In(OY323) and leu-3 (12 to 18%) (57, 808, 816, PB). (335, 1135) Cannot use nitrate, nitrite, purines, or most amino acids as a nitrogen source but will grow on ammonia, glutamine, or glutamate. nit-2+ is a major nitrogen control gene and mediates nitrogen catabolite repression. The nit-2 mutant is missing (or has severely reduced levels of) nitrate reductase, nitrite reductase, uricase, xanthine dehydrogenase, allantoinase, allantoicase, L-amino acid oxidase, general amino acid permease, extracellular protease, and an intracellular neutral phenylmethylsulfonyl fluoride-sensitive protease (227, 324, 441, 872, 1001, and references therein). Also affects levels of glutamate dehydrogenases (226) and uptake of uracil and uridine (128). Prevents leaky growth of the mutant am on minimal medium (155). The product of the nit-2gene has been tentatively identified as a nuclear DNA-binding protein, whose affinity for DNA is reduced in the presence of glutamine (433). Allele K31 (called pink) originated in N. sitophila and was introgressed into N. crassa (335); protein product of K31 may show altered mobility (433). Recombination within the nit-2 locus is subject to regulation by rec-1 (157). Heterozygosity for closely linked ss reduces recombination within nit-2 (161). Called amr: ammonium regulation in reference 872.
Reference for: nit-2: 57. Barry, E. G. , and J. F. Leslie. 1982. An interstitial pericentric inversion in Neurospora. Can. J. Genet. Cytol. 24:693-703
Reference for: nit-2: 128. Buxton, F. P. , and A. Radford. 1982. Nitrogen metabolic repression of fluoropyrimidine resistance and pyrimidine uptake in N. crassa. Mol. Gen. Genet.
Reference for: nit-2: 155. Catcheside, D. E. A. 1970. Control of recombination within the nitrate-2 locus of N. crassa: an unlinked dominant gene which reduces prototroph yields. Aust. J. Biol. Sci. 23:855- 865.
Reference for: nit-2: 157. Catcheside. D. E. A. 1974. A second locus subject to recombination control by the rec-1+ gene in N. crassa. Aust. J. Biol. Sci. 27:561-573.
Reference for: nit-2: 161. Catcheside, D. E. A. 1981. Genes in Neurospora that suppress recombination when they are heterozygous. Genetics 98:55-76.
Reference for: nit-2: 226. Dantzig, A. H. , F. L. Weigman, Jr. , and A. Nason. 1979. Regulation of glutamate dehydrogenases in nit-2 and am mutants of N. crassa. J. Bacteriol. 137:1333-1339.
Reference for: nit-2: 227. Dantzig, A. H. , W. K. Zurowski, T. M. Ball, and A. Nason. 1978. Induction and repression of nitrate reductase in N. crassa. J. Bacteriol. 134:693-698.
Reference for: nit-2: 324. Facklam, T. J. , and G. A. Marzluf. 1978. Nitrogen regulation of amino acid catabolism in Neurospora crassa. Biochem. Genet. 16:343-354.
Reference for: nit-2: 335. Fincham, J. R. S. 1951. A comparative genetic study of the mating type chromosomes of two species of Neurospora. J. Genet. 50:221-229.
Reference for: nit-2: 433. Grove, G. , and G. A. Marzluf. 1981. Indentification of the product of the major regulatory gene of the nitrogen control circuit of Neurosporacrassa as a nuclear DNA-binding protein. J. Biol. Chem. 256:463-470.
Reference for: nit-2: 441. Hanson, M. A. , and G. A. Marzluf. 1975. Control of the synthesis of a single enzyme by multiple regulatory circuits in Neurospora crassa. Proc. Natl. Acad. Sci. U. S. A. 72:1240-1244.
Reference for: nit-2: 808. Perkins, D. D. , and E. G. Barry. 1977. The cytogenetics of Neurospora. Adv. Genet. 19:133-285.
Reference for: nit-2: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.
Reference for: nit-2: 872. Reinert, W. R. , and G. A. Marzluf. 1975. Genetic and metabolic control of the purine catabolic enzymes of Neurospora crassa. Mol. Gen. Genet. 139:39-55.
Reference for: nit-2: 1001. Sorger, G. J. , R. Premakumar, and E. D. Gooden. 1978. Demonstration in vitro of two intracellular inactivators of nitrate reductase fromNeurospora. Biochim. Biophys. Acta 540:33-47.
Reference for: nit-2: 1135. Whitehouse, H. L. K. 1942. Crossing-over in Neurospora. New Phytol. 41:23-62.
Reference for: nit-2: 11006. Chambers et al 1983. Curr Genet 7:51-56


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