FGSC #5956 Mating Types: - Species: Fusarium verticillioides
Genotype: Fusarium verticillioides nit1
Alleles: FKMA928
Linkage Group(s): VCG A4
Stock No. from Other Collection: FKMA928
Depositor of Strain: JFL
Strain of Opposite Mating Type: 0
Reference: Klittich and Leslie 1988. Genetics 118:417-423
Lesion: nit-1 nitrate nonutilizer-1
Enzyme Name: molybdenum cofactor - nitrate reductase and xanthine dehydrogenase
Lesion Information for Marker:
      Linkage Group of nit-1: IR
      Markers Left of nit-1: Tp(T54M94) and ad-9 (3 to 15%)
      Markers Right ofnit-1: cyh-1(6%)
Marker description or requirements:
      IR. Right of Tp(T54M94) and ad-9 (3 to 15%). Left of cyh-1(6%) (466, 496, 816). (482)Cannot use nitrate or hypoxanthine as a nitrogen source, but uses nitrite, ammonia, or amino acids (1000). Does not prevent formation or nitrate reductase apoprotein (999), but lacks the molybdenum-containing cofactor common to nitrate reductase and xanthine dehydrogenase (591, 741) (Fig. 19 and 24). The nitrate reductase in nit-1 extracts does not catalyze the complete electron transport sequence from NADPH to N03 but does catalyze the initial part of this sequence if a suitable electron acceptor (e.g., cytochrome c) is provided (999). See reference 198 for a model of interaction of nit-1 and nit-3 gene products. See references 226, 999, and 1000 for regulation.
Reference for: nit-1: 466. Holm, K. , E. Nilheden, H. G. Kolmark, and K. Burton. 1976. Genetic and enzymatic analysis of a glycerol kinase deficient mutant inNeurospora crassa. Mol. Gen. Genet. 144:11-15.
Reference for: nit-1: 482. Houlahan, M. B. , G. W. Beadle, and H. G. Calhoun. 1949. Linkage studies with biochemical mutants of Neurospora crassa. Genetics 34:493-507.
Reference for: nit-1: 496. Hsu, K. S. 1963. The genetic basis of actidione resistance in Neurospora. J. Gen. Microbiol. 32:341-347.
Reference for: nit-1: 591. Lee, K. -Y. , S. -S. Pan, R. Erickson, and A. Nason. 1974. Involvement of molybdenum and iron in the in vitro assembly of assimilatory nitratereductase utilizing Neurospora mutant nit-1 . J. Biol. Chem. 249:3941-3952.
Reference for: nit-1: 741. Nason, A. , K. -Y. Lee, S. -S. Pan, P. A. Ketchum, A. Lamberti, and J. De Vries. 1971. In vitro formation of assimilatory reduced nicotinamideadenine dinucleotide phosphate:nitrate reductase from a Neurospora mutant and a component of molybdenum-enzymes. Proc. Natl. Acad. Sci. U. S. A. 68:3242-3246.
Reference for: nit-1: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.
Reference for: nit-1: 999. Sorger, G. J. 1966. Nitrate reductase electron transport systems in mutant and in wild-type strains of Neurospora. Biochim. Biophys. Acta 118:484-494.
Reference for: nit-1: 1000. Sorger, G. J. , and N. H. Giles. 1965. Genetic control of nitrate reductase in Neurospora crassa. Genetics 52:777-788.


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