FGSC #4143 Mating Types: A Species: Neurospora crassa
Genotype: thi-3 met-7 nt
Alleles: 18558 4894 65001
Linkage Group(s): VIIL VIIR
Stock No. from Other Collection: M670 A
Depositor of Strain: EK
Strain of Opposite Mating Type: 4144
Reference: Kafer 1982. Neurospora Newsl. 29:41-44
Markers
Lesion: met-7 methionine-7
Enzyme Name: cystathionine-g-synthase
Lesion Information for Marker:
      Linkage Group of met-7: VIIR
      Markers Left of met-7: qa-2 (<1%), ars (<1%), and the centromere
      Markers Right ofmet-7: met-9 and wc-1(1-4%)
Marker description or requirements:
      VIIR. Right of qa-2 (<1%), ars (<1%), and the centromere (one second-division ascus in several hundred). Left of met-9 (10[-4]) and wc-1 (1 to 4%) (146, 725; M.E. Case, personal communication). (718; M.K. Allen, cited in references 718 and 789) Uses cystathionine, homocysteine, or methionine (718; N. H. Horowitz, cited in reference 1180). Lacks cystathionine-gamma-synthase (547) (Fig. 17). This enzyme is also lacking in the mutant met-3 (547). See met-3 for regulation. Apparently contiguous with met-9by coconversion. Flanking markers are recombined in most met-7+ met-9+ recombinants (725). Functionally distinct from the mutant met-9, which has active cystathionine-gamma-synthase (547) but cannot use homocysteine. No mutants lacking both functions have been isolated. Allele NM251 is suppressible by supersuppressor RN33 (same as ssu-1?) (725). Allele K79 is inseparable from reciprocal translocation T(I;VII)K79 (808).
Reference for: met-7: 146. Case. M. E. , and N. H. Giles. 1976. Gene order in the qa gene cluster of N. crassa. Mol. Gen. Genet. 147:83-89.
Reference for: met-7: 547. Kerr, D. S. , and M. Flavin. 1970. The regulation of methionine synthesis and the nature of cystathionine-synthase in Neurospora. J. Biol. Chem. 245:1842-1855.
Reference for: met-7: 718. Murray, N. E. 1960. The distribution of methionine loci in Neurospora crassa. Heredity 15:199-206.
Reference for: met-7: 725. Murray, N. E. 1970. Recombination events that span sites within neighbouring gene loci of Neurospora. Genet. Res. 15:109-121.
Reference for: met-7: 789. Perkins, D. D. 1959. New markers and multiple point linkage data in Neurospora. Genetics 44:1185-1208.
Reference for: met-7: 808. Perkins, D. D. , and E. G. Barry. 1977. The cytogenetics of Neurospora. Adv. Genet. 19:133-285.
Reference for: met-7: 1180. Zalokar, M. 1950. The sulfanomide-requiring mutant of Neurospora:threonine-methionine antagonism. J. Bacteriol. 60:191-203.
Lesion: nt nicotinic acid or tryptophan
Enzyme Name: tryptophan pyrrolase
Lesion Information for Marker:
      Linkage Group of nt: VIIR
      Markers Left of nt: arg-10 (2 to 12%)
      Markers Right ofnt: sk (7 to 18%)
Marker description or requirements:
      VIIR. Between arg-10 (2 to 12%) and sk (7 to 18%) (789). (874) Uses nicotinic acid. May respond also to tryptophan, phenylalanine, tyrosine, quinic acid, and precursors of nicotinic acid or tryptophan, or both, depending on genetic background (448, 760). Best supplemented with nicotinamide and scored as a nic mutant. Probably deficient in tryptophan pyrrolase (tryptophan 2,3-dioxygenase) (Fig. 18), but direct evidence is lacking because tryptophan oxygenase cannot be assayed in Neurospora (368). Kynurenine formamidase levels are normal (368). Pyridine nucleotide levels (111).
Reference for: nt: 111. Brody, S. 1972. Regulation of pyridine nucleotide levels and ratios in Neurospora crassa. J. Biol. Chem. 247:6013-6017.
Reference for: nt: 368. Gaertner, F. H. 1981. A response to a letter from David Perkins concerning the question of whether nt mutants involve tryptophanpyrrolase. Neurospora Newsl. 28:12.
Reference for: nt: 448. Haskins, F. H. , and H. K. Mitchell. 1952. An example of the influence of modifying genes in Neurospora. Am. Nat. 86:231-238.
Reference for: nt: 760. Newmeyer, D. , and E. L. Tatum. 1953. Gene expression in Neurospora mutants requiring nicotinic acid or tryptophan. Am. J. Bot. 40:393-400.
Reference for: nt: 789. Perkins, D. D. 1959. New markers and multiple point linkage data in Neurospora. Genetics 44:1185-1208.
Reference for: nt: 874. Reisner, A. , R. W. Barratt, and D. Newmeyer. 1953. Confirmation of the seventh linkage group of Neurospora crassa. Genetics 38:685(Abstr. ).
Lesion: thi-3 thiamine-3
Lesion Information for Marker:
      Linkage Group of thi-3: VIIL
      Markers Left of thi-3: nic-3 (9 to 18%)
      Markers Right ofthi-3: T(T54M50)
Marker description or requirements:
      VIIL. Between nic-3 (9 to 18%) and T(T54M50) (808, 812, 816). (482). Uses thiamine or thiazole (1059). Does not undergo growth adaptation on minimal medium. Growth on minimal medium is leaky at first, but becomes tight with exhaustion of endogenous thiazole (302), so scoring is best done late.
Reference for: thi-3: 302. Eberhart, B. M. , and E. L. Tatum. 1961. Thiamine metabolism in Neurospora crassa. Am. J. Bot. 48:702-711.
Reference for: thi-3: 482. Houlahan, M. B. , G. W. Beadle, and H. G. Calhoun. 1949. Linkage studies with biochemical mutants of Neurospora crassa. Genetics 34:493-507.
Reference for: thi-3: 808. Perkins, D. D. , and E. G. Barry. 1977. The cytogenetics of Neurospora. Adv. Genet. 19:133-285.
Reference for: thi-3: 812. Perkins, D. D. , M. Glassey, and B. A. Bloom. 1962. New data on markers and rearrangements in Neurospora. Can. J. Genet. Cytol. 4:187-205.
Reference for: thi-3: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.
Reference for: thi-3: 1059. Tatum, E. L. , and T. T. Bell. 1946. Neurospora. III. Biosynthesis of thiamin. Am. J. Bot. 33:15-20.

 

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