FGSC #3632 Mating Types: a Species: Neurospora crassa
Genotype: fl;inl
Alleles: P;37401
Linkage Group(s): IIR;VR
Stock No. from Other Collection: 9282
Depositor of Strain: DDP
Strain of Opposite Mating Type: 0
Lesion: fl fluffy
Lesion Information for Marker:
      Linkage Group of fl: IIR
      Markers Left of fl: ace-1 (5 to 11%)
      Markers Right offl: trp-3 (3%)
Marker description or requirements:
      IIR. Between ace-1 (5 to 11%) and trp-3 (3%) (816, PB). (613)No macroconidia (609). Highly fertile (612). Used routinely as the female parent in tests for chromosome rearrangements and for mating type (e.g., reference 801). The flsingle mutant produces few microconidia when dry; when wetted, sufficient microconidia are produced to have been used in early irradiation and mutation studies (614, 915); large numbers can be obtained under certain conditions; see reference 893. pe fl (46, 700) and fl;dn (806) double mutants produce abundant microconidia; the latter combination is highly fertile when homozygous. Photograph of microconidial formation (774); see also reference 893. Nuclear numbers in microconidia (46, 64, 478). Wall analysis (207). Immunoelectrophoretic pattern (784). Paradoxical high alcoholic glycolysis on nitrate medium (80). Deficiency of isocitrate lyase on acetate medium; see citations in reference 1088. When fl A and fl a strains are inoculated separately on crossing medium in plates, a double line of perithecia forms where they meet, similar to that accompanying barrage in Podospora (410, 414). fl ascospores from certain fl x fl+ crosses often germinate spontaneously (1127; N. B. Raju, personal communication). Allele C-1835 was called acon (717, 812).
Reference for: fl: 46. Barratt, R. W. and L. Garnjobst. 1949. Genetics of a colonial microconidiating mutant strain of N. crassa. Genetics 34:351-369.
Reference for: fl: 64. Baylis, J. R. , Jr. , and A. G. DeBusk. 1967. Estimation of the frequency of multinucleate conidia in microconidiating strains. NeurosporaNewsl. 11:9.
Reference for: fl: 80. Bernardini, D. , and G. Turian. 1978. Recherches sur la differenciation conidienne de N. crassa. VII. Regulation alcooligene et capacite deconidiation (souche sauvage et mutant "fluffy"). Ann Microbiol. (Paris) 129B:551-559.
Reference for: fl: 207. Coniordos, N. , and G. Turian. 1973. Recherches sur la differenciation conidienne de N. crassa. IV. Modifications chimio-structurales de laparoi chez le type sauvage et chez deux mutants aconidiens. Ann. Microbiol. (Paris) 124A:5-28.
Reference for: fl: 410. Griffiths, A. J. F. 1979. A Neurospora experiment for an introductory biology course. Neurospora Newsl. 26:12.
Reference for: fl: 414. Griffiths, A. J. F. , and A. Rieck. 1981. Perithecial distribution patterns in standard and variant strains of Neurospora crassa. Can. J. Bot. 59:2610-2617,.
Reference for: fl: 478. Horowitz, N. H. , and H. Macleod. 1960. The DNA content of Neurospora nuclei. Microb. Genet. Bull. 17:6-7.
Reference for: fl: 609. Lindegren, C. C. 1933. The genetics of Neurospora. III. Pure bred stocks and crossing-over in N. crassa. Bull. Torrey Bot. Club 60:133-154.
Reference for: fl: 612. Lindegren, C. C. , V. Beanfield, and R. Barber. 1939. Increasing the fertility of Neurospora by selective inbreeding. Bot. Gaz. 100:592-599.
Reference for: fl: 613. Lindegren, C. C. , and G. Lindegren. 1939. Non-random crossing-over in the second chromosome of Neurospora crassa. Genetics 24:1-7.
Reference for: fl: 614. Lindegren, C. C. , and G. Lindegren. 1941. X-ray and ultraviolet induced mutations in Neurospora. I. X-ray mutations. J. Hered. 32:404-412.
Reference for: fl: 700. Munkres, K. D. 1977. Selection of improved microconidial strains of Neurospora crassa. Neurospora Newsl. 24:9-10.
Reference for: fl: 717. Murray, J. C. , and A. M. Srb. 1962. The morphology and genetics of wild-type and seven morphological mutant strains of Neurosporacrassa. Can. J. Bot. 40:337-350.
Reference for: fl: 774. Oulevey-Matikian, N. , and G. Turian. 1968. Contrle metabolique et aspects ultrastructuraux de la conidiation (macro-microconidies) deNeurospora crassa. Arch. Mikrobiol. 60:35-58.
Reference for: fl: 784. Peduzzi, R. , and G. Turian. 1972. Recherches sur la differenciation conidienne de Neurospora crassa. III. Activitemalico-deshydrogenasique de structures antigeniques et ses relations avec la competence conidienne. Ann. Inst. Pasteur 122:1081-1097.
Reference for: fl: 801. Perkins, D. D. 1974. The manifestation of chromosome rearrangements in unordered asci of Neurospora. Genetics 77:459-489.
Reference for: fl: 806. Perkins, D. D. 1979. A new, highly fertile microconidiating combination, dingy, fluffy. Neurospora Newsl. 26:9.
Reference for: fl: 812. Perkins, D. D. , M. Glassey, and B. A. Bloom. 1962. New data on markers and rearrangements in Neurospora. Can. J. Genet. Cytol. 4:187-205.
Reference for: fl: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.
Reference for: fl: 915. Sansome, E. R. , M. Demerec, and A. Hollaender. 1945. Quantitative irradiation experiments with Neurospora crassa. I. Experiments withX-rays. Am. J. Bot. 32:218-226.
Reference for: fl: 1127. Weijer, J. 1954. A genetical investigation into the td-locus of Neurospora crassa. Genetica 28:173-252.
Lesion: inl inositol
Enzyme Name: myoinositol-1-phosphatase
Lesion Information for Marker:
      Linkage Group of inl: VR
      Markers Left of inl: pho-3 (3 to 4%),and al-3
      Markers Right ofinl: pab-1 (1 to 10%)
Marker description or requirements:
      VR. Between pho-3 (3 to 4%) and pab-1 (1 to 10%). Right of al-3 (362, 397, 1036). (482)Requires inositol (65). Lacks D-myoinositol-1-phosphatase (1142). Lack of glucocycloaldolase found by Pina and Tatum (826) is attributed by Williams (1142) to drastic repression of glucocycloaldolase by the concentration of inositol used for growth. Growth is colonial on low levels of inositol (367). Tends to extrude dark pigment into the medium when grown on suboptimal inositol. Composition of phospholipids and cell walls is abnormal on limiting inositol (367, 439, 440, 501). Inhibited by hexachlorocyclohexane (366, 457, 931). Conidia are subject to death by unbalanced growth on minimal medium (1028, 1033), a property exploited for mutant enrichment ("inositol-less death") (606, 647) because double mutants are at a selective advantage. Heat-sensitive allele 83201 is especially useful for mutant enrichment (832, 1043). Used in the first experiments reporting transformation of Neurospora by N. crassaDNA (677, 679) and reported to be efficient as a recipient in absence of inositol (1162). Used to study glucose (917) and sulfate (641) transport systems. Used extensively for studying induced reversion (392). Used for studying the mechanism of inositol-less death (647, 702), mutagenicity of ferrous ions, and regulation of mitochondrial membrane fluidity; for a review, see reference 702. Spontaneous reversion rates (386). Allele-specific partial suppressor (390). Allele 46802 is nonrevertable and inseparable from translocation 46802 (386, 808). Strains carrying heat-sensitive allele 83201 show slow semicolonial growth in liquid minimal medium at 25°C (641), but look normal on slants (D.D. Perkins, unpublished data). Strains carrying allele 89601 contain cross-reacting material (1183). Mutant gene exo-1 is present in the inl(89601) a stock FGSC 498 and may, therefore, be present in stocks of mutants derived by inositol-less death. (See references 194, 325, and 1027). Called inos.
Reference for: inl: 65. Beadle, G. W. 1944. An inositolless mutant strain of Neurospora and its use in bioassays. J. Biol. Chem. 156:683-689.
Reference for: inl: 194. Chen, Y. F. , C. C. Ho, and A. L. Demain. 1976. Genetic improvement of production of amylases in Neurospora intermedia. Genetics 83:s14(Abstr).
Reference for: inl: 325. Fass, D. N. 1969. Glucamylase of Neurospora:a regulated exoenzyme. Ph. D. thesis, Florida State University. Diss. Abstr. Intern. 30:5375B(1970).
Reference for: inl: 362. Freese, E. 1957. Uber die Feinstruktur des Genoms im Bereich eines PAB Locus von Neurospora crassa. Z. Indukt. Abstamungs. -Vererbungsl. 88:388-406.
Reference for: inl: 366. Fuller, R. C. , R. W. Barratt, and E. L. Tatum. 1950. The relationship between hexachlorocyclohexane and inositol in Neurospora. J. Biol. Chem. 186:823-827.
Reference for: inl: 367. Fuller, R. C. , and E. L. Tatum. 1956. Inositol-phospholipid in Neurospora and its relationship to morphology. Am. J. Bot. 43:361-365.
Reference for: inl: 386. Giles, N. H. 1951. Studies on the mechanism of reversion in biochemical mutants of Neurospora crassa. Cold Spring Harbor Symp. Quant. Biol. 16:283-313.
Reference for: inl: 390. Giles, N. H. , and C. W. H. Partridge. 1953. The effect of a suppressor on allelic inositolless mutants in Neurospora crassa. Proc. Natl. Acad. Sci. U. S. A. 39:479-488.
Reference for: inl: 392. Giles, N. H. , F. J. de Serres, and C. W. H. Partridge. 1955. Comparative studies of X-ray-induced forward and reverse mutation. Ann. N. Y. Acad. Sci. 59:536-552.
Reference for: inl: 397. Gleason, M. K. , and R. L. Metzenberg. 1974. Regulation of phosphate metabolism in Neurospora crassa:isolation of mutants deficient inthe repressible alkaline phosphatase. Genetics 78:645-659.
Reference for: inl: 439. Hanson, B. A. 1980. Inositol-limited growth, repair, and translocation in an inositol-requiring mutant of Neurospora crassa. J. Bacteriol. 143:18-26.
Reference for: inl: 440. Hanson, B. , and S. Brody. 1979. Lipid and cell wall changes in an inositol-requiring mutant of Neurospora crassa. J. Bacteriol. 138:461-466.
Reference for: inl: 457. Hitz, H. -R. 1963. Die Wirkung von Hexachlorcyclohexanen bei Neurospora crassa inositolless. Arch. Mikrobiol. 45:217-246.
Reference for: inl: 482. Houlahan, M. B. , G. W. Beadle, and H. G. Calhoun. 1949. Linkage studies with biochemical mutants of Neurospora crassa. Genetics 34:493-507.
Reference for: inl: 501. Hubbard, S. C. , and S. Brody. 1975. Glycerophospholipid variation in choline and inositol auxotrophs of Neurospora crassa:internalcompensation among zwitterionic and anionic species. J. Biol. Chem. 250:7173-7181.
Reference for: inl: 606. Lester, H. E. , and S. R. Gross. 1959. Efficient method for selection of auxotrophic mutants of Neurospora. Science 129:572.
Reference for: inl: 641. Marzluf, G. A. 1973. Regulation of sulfate transport in Neurospora by transinhibition and by inositol depletion. Arch. Biochem. Biophys. 156:244-254.
Reference for: inl: 647. Matile, P. 1966. Inositol deficiency resulting in death:an explanation of its occurrence in Neurospora crassa. Science 151:86- 88.
Reference for: inl: 677. Mishra, N. C. , G. Szabo, and E. L. Tatum. 1973. Nucleic acid-induced genetic changes in Neurospora. In M. C. Niu and S. J. Segal (ed. ), The role of RNA in reproduction and development. North/Holland, Amsterdam.p. 259-268.
Reference for: inl: 679. Mishra, N. C. , and E. L. Tatum. 1973. Non-Mendelian inheritance of DNA-induced inositol independence in Neurospora. Proc. Natl. Acad. Sci. U. S. A. 70:3875-3879.
Reference for: inl: 702. Munkres, K. D. 1981. Biochemical genetics of aging of Neurospora crassa and Podospora anserina:a review. In R. S. Sohal (ed. ),Age pigments. North-Holland, Amsterdam.p. 83-100.
Reference for: inl: 808. Perkins, D. D. , and E. G. Barry. 1977. The cytogenetics of Neurospora. Adv. Genet. 19:133-285.
Reference for: inl: 826. Pina, E. , and E. L. Tatum. 1967. Inositol biosynthesis in Neurospora crassa. Biochim. Biophys. Acta 136:265-271.
Reference for: inl: 832. Pittenger, T. H. , and D. J. West. 1979. Isolation and characterization of temperature-sensitive respiratory mutants of Neurospora crassa. Genetics 93:539-555.
Reference for: inl: 917. Sargent, M. L. , and S. H. Kaltenborn. 1972. Effects of medium composition and carbon dioxide on circadian conidiation in Neurospora. Plant Physiol. 50:171-175.
Reference for: inl: 931. Schopfer, W. H. , and T. Posternak. 1958. Action d'anti-inositols sur Neurospora crassa "inositolless" cultive en milieu hautement purifie. Arch. Mikrobiol. 31:240-243.
Reference for: inl: 1027. Sternberg, D. , and A. S. Sussman. 1974. Hyperproduction of some glycosidases in Neurospora crassa. Arch. Microbiol. 101:303-320.
Reference for: inl: 1028. Stevens, C. M. , and A. Mylroie. 1953. Inhibition effects in back-mutation tests with mutants of Neurospora. Nature 171:179-180.
Reference for: inl: 1033. Strauss, B. S. 1958. Cell death and "unbalanced growth" in Neurospora. J. Gen. Microbiol. 18:658-669.
Reference for: inl: 1036. Strickland, W. N. , D. D. Perkins, and C. C. Veatch. 1959. Linkage data for group V markers in Neurospora. Genetics 44:1221-1226.
Reference for: inl: 1043. Sullivan, J. L. , and A. G. DeBusk. 1971. Method for specific selection of temperature-sensitive mutants. Neurospora Newsl. 18:13.
Reference for: inl: 1142. Williams, S. G. 1971. Biosynthesis of inositol by inositol-less mutants of Neurospora crassa. Aust. J. Biol. Sci. 24:1181-1188.
Reference for: inl: 1162. Wootton, J. C. , M. J. Fraser, and A. J. Baron. 1980. Efficient transformation of germinating Neurospora conidia using total nuclear DNAfragments. Neurospora Newsl. 27:33.
Reference for: inl: 1183. Zsindely, A. , M. Szabolcs, M. Kavai, M. Schablik, J. Aradi, and G. Szabo. 1979. Demonstration of myo-inositol1-phosphate synthase andits assumed defective variant in various Neurospora crassa strains by immunological methods. Acta Biol. Acad. Sci. Hung. 30:141-149.


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