FGSC #3607 Mating Types: A Species: Neurospora crassa
Genotype: met-7 met-9 wc-1
Alleles: NM331 NM43(t) P829
Linkage Group(s): VIIR R R
Stock No. from Other Collection: #7102
Depositor of Strain: AR
Strain of Opposite Mating Type: 0
Reference: Murray, N. 1970 Genetical Research 15:109-121
Markers
Lesion: met-7 methionine-7
Enzyme Name: cystathionine-g-synthase
Lesion Information for Marker:
      Linkage Group of met-7: VIIR
      Markers Left of met-7: qa-2 (<1%), ars (<1%), and the centromere
      Markers Right ofmet-7: met-9 and wc-1(1-4%)
Marker description or requirements:
      VIIR. Right of qa-2 (<1%), ars (<1%), and the centromere (one second-division ascus in several hundred). Left of met-9 (10[-4]) and wc-1 (1 to 4%) (146, 725; M.E. Case, personal communication). (718; M.K. Allen, cited in references 718 and 789) Uses cystathionine, homocysteine, or methionine (718; N. H. Horowitz, cited in reference 1180). Lacks cystathionine-gamma-synthase (547) (Fig. 17). This enzyme is also lacking in the mutant met-3 (547). See met-3 for regulation. Apparently contiguous with met-9by coconversion. Flanking markers are recombined in most met-7+ met-9+ recombinants (725). Functionally distinct from the mutant met-9, which has active cystathionine-gamma-synthase (547) but cannot use homocysteine. No mutants lacking both functions have been isolated. Allele NM251 is suppressible by supersuppressor RN33 (same as ssu-1?) (725). Allele K79 is inseparable from reciprocal translocation T(I;VII)K79 (808).
Reference for: met-7: 146. Case. M. E. , and N. H. Giles. 1976. Gene order in the qa gene cluster of N. crassa. Mol. Gen. Genet. 147:83-89.
Reference for: met-7: 547. Kerr, D. S. , and M. Flavin. 1970. The regulation of methionine synthesis and the nature of cystathionine-synthase in Neurospora. J. Biol. Chem. 245:1842-1855.
Reference for: met-7: 718. Murray, N. E. 1960. The distribution of methionine loci in Neurospora crassa. Heredity 15:199-206.
Reference for: met-7: 725. Murray, N. E. 1970. Recombination events that span sites within neighbouring gene loci of Neurospora. Genet. Res. 15:109-121.
Reference for: met-7: 789. Perkins, D. D. 1959. New markers and multiple point linkage data in Neurospora. Genetics 44:1185-1208.
Reference for: met-7: 808. Perkins, D. D. , and E. G. Barry. 1977. The cytogenetics of Neurospora. Adv. Genet. 19:133-285.
Reference for: met-7: 1180. Zalokar, M. 1950. The sulfanomide-requiring mutant of Neurospora:threonine-methionine antagonism. J. Bacteriol. 60:191-203.
Lesion: met-9 methionine-9
Lesion Information for Marker:
      Linkage Group of met-9: VIIR
      Markers Left of met-9: met-7 (10[-4])
      Markers Right ofmet-9: wc-1 (1 to 2%)
Marker description or requirements:
      VIIR. Between met-7 (10[-4]) and wc-1 (1 to 2%) (725). (815) Requires methionine; cannot use precursors (290, 718, 725). Apparently contiguous with met-7, q.v. (725). Functionally distinct from met-7. The mutant met-9 retains the met-7+ function, producing cystathionine-gamma-synthase (547). Allele NM43 is heat sensitive (725).
Reference for: met-9: 290. Dubes, G. R. 1953. Investigations of some "unknown" mutants of Neurospora crassa. Ph. D. thesis, California Institute of Technology.
Reference for: met-9: 547. Kerr, D. S. , and M. Flavin. 1970. The regulation of methionine synthesis and the nature of cystathionine-synthase in Neurospora. J. Biol. Chem. 245:1842-1855.
Reference for: met-9: 718. Murray, N. E. 1960. The distribution of methionine loci in Neurospora crassa. Heredity 15:199-206.
Reference for: met-9: 725. Murray, N. E. 1970. Recombination events that span sites within neighbouring gene loci of Neurospora. Genet. Res. 15:109-121.
Reference for: met-9: 815. Perkins, D. D. , and N. E. Murray. 1963. New markers and linkage data. Neurospora Newsl. 4:26-27.
Lesion: wc-1 white collar-1
Lesion Information for Marker:
      Linkage Group of wc-1: VIIR
      Markers Left of wc-1: met-9 (1 to 4%)
      Markers Right ofwc-1: un-10 (7%) and for (6%)
Marker description or requirements:
      VIIR. Right of met-9 (1 to 4%). Left of un-10 (7%) and for (6%) (724, 812, 816). Carotenoids absent from mycelia; conidia become pigmented with some delay. Named because nonconidiating rim at top of agar slant remains white. A double mutant with flor other nonconidiating mutant would be classed as albino. Regulatory mutants for photoinduced carotenogenesis via blue light receptor might be expected to have a similar phenotype (444, 445). A blue light treatment (given in vivo), which increases the activity of soluble and microsomal enzymes required for phytoene biosynthesis in the wild type, does not do so in the mutant wc-1 (445). Fails to show phototropism of perithecial beaks when used as the female (protoperithecial) parent, but not when used as the male (fertilizing) parent (R. W. Harding, personal communication). Useful genetic marker (725, 800). Scoring clearest at high temperatures (34 C).
Reference for: wc-1: 444. Harding, R. W. , and W. Shropshire, Jr. 1980. Photocontrol of carotenoid biosynthesis. Annu. Rev. Plant Physiol. 31:217-238.
Reference for: wc-1: 445. Harding, R. W. , and R. V. Turner. 1981. Photoregulation of the carotenoid biosynthetic pathway in albino and white collar mutants ofNeurospora crassa. Plant Physiol. 68:745-749.
Reference for: wc-1: 724. Murray, N. E. 1969. Reversal of polarized recombination of alleles in Neurospora as a function of their position. Genetics 61:67-77.
Reference for: wc-1: 725. Murray, N. E. 1970. Recombination events that span sites within neighbouring gene loci of Neurospora. Genet. Res. 15:109-121.
Reference for: wc-1: 800. Perkins, D. D. 1972. Linkage testers having markers near the centromere. Neurospora Newsl. 19:33.
Reference for: wc-1: 812. Perkins, D. D. , M. Glassey, and B. A. Bloom. 1962. New data on markers and rearrangements in Neurospora. Can. J. Genet. Cytol. 4:187-205.
Reference for: wc-1: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.

 

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6/11/12