FGSC #A518 Species: Aspergillus nidulans
Translocations: T1(VR.VIR)
Genotype: AcrA1;lysB5 facA303 hxA2;lacA1 sB3 sbA3 T1(V;VI)
Linkage Group(s): IIL;VL VR VR;VIR VIR VIR
Genetic Background: cro
Stock No. from Other Collection: M2318
Depositor of Strain: EK
Strain of Opposite Mating Type: 0
Reference: Kafer 1977. Adv in Genet 19:33-131
Markers
Lesion: acrA1 acriflavine
Lesion Information for Marker:
      Linkage Group of acrA1: IIL
Marker description or requirements:
      Resistant to acriflavine (25-50 ug/ml)
Reference for: acrA1: 10568. Roper, J. A. & E. Kafer 1957. J. Gen. Microbiol. 16,660.
Lesion: facA303 fluoroacetate res., acetate ut. acetyl-coA synthetase
Enzyme Name: acetyl CoA synthetase
Lesion Information for Marker:
      Linkage Group of facA303: VR
Marker description or requirements:
      Resistant to fluoroacetate, cannot use acetate as a carbon source
Reference for: facA303: 10005. Aleksenko, A. . unpublished
Reference for: facA303: 10024. Armitt, S. , W. McCullough & C. F. Roberts 1976. J. Gen. Microbiol. 92;263-282
Reference for: facA303: 10182. Connerton, I. F. , J. R. S. Fincham, R. A. Sandeman & M. J. Hynes 1990. Mol. Microbiol. 4,451-460
Reference for: facA303: 10204. Davis, M. A. & M. J. Hynes 1989. Trends Genet. 5;14-19
Reference for: facA303: 10582. Sandeman, R. A. & M. J. Hynes 1989. Mol. Gen. Genet. 218,87-92
Lesion: hxA2 hypoxanthine non-ut., xanthine deH I
Enzyme Name: xanthine dehydrogenase I
Lesion Information for Marker:
      Linkage Group of hxA2: VR
Marker description or requirements:
      cannot use hypoxanthine as a nitrogen source
Reference for: hxA2: 10200. Darlington, A. J. , C. Scazzocchio & J. A. Pateman 1965. Nature 206,599.
Reference for: hxA2: 10204. Davis, M. A. & M. J. Hynes 1989. Trends Genet. 5;14-19
Reference for: hxA2: 10292. Holl, F. B. & C. Scazzocchio 1970. FEBS Letters 12,51.
Reference for: hxA2: 10590. Scazzocchio, C. & G. Gorton 1977. In ref. 626. pp255-265.
Lesion: lacA1 lactose ut.
Lesion Information for Marker:
      Linkage Group of lacA1: VIR
Marker description or requirements:
      Forms normal size but diffuse colonies when lactose is sole C source
Reference for: lacA1: 10556. Roberts, C. F. 1963. J. Gen. Microbiol. 31,45.
Lesion: lysB5 lysine req.
Lesion Information for Marker:
      Linkage Group of lysB5: VL
Marker description or requirements:
      requires lysine, slight response to aminoadipate
Reference for: lysB5: 10512. Pees, E. 1966. Aspergillus News Letter 7,11.
Reference for: lysB5: 10536. Pontecorvo, G. et al. 1953. Adv. Genet. 5,141-238
Lesion: sB3 sulphate ut., sulphate upt
Enzyme Name: deficient in sulfate uptake
Lesion Information for Marker:
      Linkage Group of sB3: VIR
Marker description or requirements:
      resistant to 10 mM chromate, 1 mM selenate
Reference for: sB3: 10026. Arst, H. N. jr. 1968. Nature, 219,268
Reference for: sB3: 10266. Gravel, R. A. , et al. 1970. Can. J. Genet. Cytol. 12,831.
Reference for: sB3: 10332. Kafer, E. 1958. Adv. Genet. , 9,105.
Lesion: sbA3 sulphate ut., sulphate upt
Lesion Information for Marker:
      Linkage Group of sbA3: VIR
Marker description or requirements:
      Poor growth, as measured colony size vs wt on plates, on sorbitol as sole C source
Reference for: sbA3: 10556. Roberts, C. F. 1963. J. Gen. Microbiol. 31,45.
Lesion: T1(V;VI) translocation breakpoint
Lesion Information for Marker:
      Linkage Group of T1(V;VI): V,VI
Marker description or requirements:
      Translocation breakpoint
Reference for: T1(V;VI): 10338. Kafer, E. 1977. Adv. Genet. 19,33-131.

 

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6/11/12