FGSC #3427 Mating Types: a Species: Neurospora crassa
Genotype: cyb-3;his-6
Alleles: A10;Y175M16
Linkage Group(s): IIL;VR
Stock No. from Other Collection: Y175M616
Depositor of Strain: DVW
Strain of Opposite Mating Type: 0
Reference: West & Pittenger Molec gen Genet 152:77-82 1977
Markers
Lesion: cyb-3 cytochrome b-3
Lesion Information for Marker:
      Linkage Group of cyb-3: IIL
      Markers Right ofcyb-3: ro-3 (9%)
Marker description or requirements:
      IIL. Left of ro-3 (9%) (PB). Deficient in cytochrome b. Cannot reduce tetrazolium. Slow growth. Heat sensitive: mutant phenotype at 38 to 39°C; nearly normal at 25 and 34°C (1133). Grows slowly from ascospores at 34°C.
Reference for: cyb-3: 1133. West, D. J. , and T. H. Pittenger. 1977. A temperature-sensitive mutant of Neurospora crassa deficient in cytochrome b. Mol. Gen. Genet. 152:77-82.
Lesion: his-6 histidine-6
Lesion Information for Marker:
      Linkage Group of his-6: VR
      Markers Left of his-6: un-9 (6%)
      Markers Right ofhis-6: pyr-6 (6 to 18%)
Marker description or requirements:
      VR. Right of un-9 (6%) and pyr-6 (6 to 18%). No recombination with terminal translocation T(NMI49) (793, 808, 816, 818, PB). Report of IV linkage (646) not confirmed.Requires histidine. Blocked before imidazole glycerol phosphate (162, 1123) (Fig. 14). No intralocus complementation (95 alleles) (162). Intralocus recombination (172).
Reference for: his-6: 162. Catcheside, D. G. 1960. Complementation among histidine mutants of N. crassa. Proc. R. Soc. Lond. B Biol. Sci. 153:179-194.
Reference for: his-6: 172. Catcheside, D. G. , and B. Austin. 1969. The control of allelic recombination at histidine loci in N. crassa. Am. J. Bot. 56:685-690.
Reference for: his-6: 646. Mathieson, M. J. , and D. G. Catcheside. 1955. Inhibition of histidine uptake in Neurospora crassa. J. Gen. Microbiol. 13:72-83.
Reference for: his-6: 793. Perkins, D. D. 1966. Summary of markers at extremes of the known linkage groups. Neurospora Newsl. 9:12.
Reference for: his-6: 808. Perkins, D. D. , and E. G. Barry. 1977. The cytogenetics of Neurospora. Adv. Genet. 19:133-285.
Reference for: his-6: 816. Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett. 1969. New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica 40:247-278.
Reference for: his-6: 818. Perkins, D. D. , M. R. Smith, and D. R. Galeazzi. 1973. New markers and linkage data. Neurospora Newsl. 20:45-49.
Reference for: his-6: 1123. Webber, B. B. , and M. E. Case. 1960. Genetical and biochemical studies of histidine-requiring mutants of Neurospora crassa. I. Classification of mutants and characterization of mutant groups. Genetics 45:1605-1615.

 

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6/11/12